Journal of Diagnostics Concepts & Practice >

2019 , Vol. 18 >Issue 05: 532 - 537

DOI: https://doi.org/10.16150/j.1671-2870.2019.05.009

Original articles

Study on in vitro biofilm formation ability of Acinetobacter baumannii

Expand
  • Department of Laboratory Medicine, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China

Received date: 2019-02-21

  Online published: 2019-10-25

Fold

Abstract

Objective: To study the in vitro biofilm formation ability of Acinetobacter baumannii for providing a reference for clinical prevention and treatment of Acinetobacter baumannii infection and control of its biofilm formation. Methods: One hundred and one strains of Acinetobacter baumannii isolated from infected patients at Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine from October to December 2017 were collected. The ability of the strains to form biofilm at different temperatures was detected by crystal violet staining; the antimicrobial susceptibility test was performed by Vitek2 Compact and the disc diffusion method. The carrying status of biofilm formation-related genes (bap, abaI, bfmS, csuAB, csuA, csuC, csuD, csuE, cpaA, and ompA) was detected by PCR. Results: The positive rate of Acinetobacter baumannii biofilm formation at 25 ℃ (93.1%) was significantly higher than that at 35 ℃ (60.4%) (P<0.05). There was a correlation between the resistance rate of isolates to Ampicillin/Sulbactam or Aminoglycosides and the biofilm formation ability (P<0.05); and there was no significant correlation between the biofilm formation ability and whether it carried the abovementioned 10 related genes (P>0.05). Conclusions: The biofilm formation ability of Acinetobacter baumannii is related to the culture temperature, and also correlated in some degree with the resistance rate to Ampicillin/sulbactam and Aminoglycosides. However, the biofilm formation ability is not related to the abovementioned 10 related genes (bap, abaI, etc.).

Key words： Acinetobacter baumannii; Biofilm; Crystal violet staining

Cite this article

HUANGFU Yuchan, DIAO Wenjing, YU Jing, LIU Ying, SHEN Lisong . Study on in vitro biofilm formation ability of Acinetobacter baumannii[J]. Journal of Diagnostics Concepts & Practice, 2019 , 18(05) : 532 -537 . DOI: 10.16150/j.1671-2870.2019.05.009

References

[1] Gonzalez-Villoria AM, Valverde-Garduno V. Antibiotic-Resistant Acinetobacter baumannii Increasing Success Remains a Challenge as a Nosocomial Pathogen[J]. J Pathog, 2016, 2016:7318075.
[2] 胡付品, 郭燕, 朱德妹, 等. 2017年CHINET中国细菌耐药性监测[J]. 中国感染与化疗杂志, 2018, 18(3):241-251.
[3] Hall-Stoodley L, Costerton JW, Stoodley P. Bacterial biofilms: from the natural environment to infectious disea-ses[J]. Nat Rev Microbiol, 2004, 2(2):95-108.
[4] Vatan A, Saltoglu N, Yemisen M, et al. Association between biofilm and multi/extensive drug resistance in diabetic foot infection[J]. Int J Clin Pract, 2018, 72(3):e13060.
[5] Babapour E, Haddadi A, Mirnejad R, et al. Biofilm formation in clinical isolates of nosocomial Acinetobacter baumannii and its relationship with multidrug resistance[J]. Asian Pac J Trop Biomed, 2016, 6(6):528-533.
[6] Tomaras AP1, Flagler MJ, Dorsey CW, et al. Characterization of a two-component regulatory system from Acinetobacter baumannii that controls biofilm formation and cellular morphology[J]. Microbiology, 2008, 154(Pt 11):3398-3409.
[7] Tomaras AP, Dorsey CW, Edelmann RE, et al. Attachment to and biofilm formation on abiotic surfaces by Acinetobacter baumannii: involvement of a novel chape-rone-usher pili assembly system[J]. Microbiology, 2003, 149(Pt 12):3473-3484.
[8] Aliramezani A, Douraghi M, Hajihasani A, et al. Clonal relatedness and biofilm formation of OXA-23-producing carbapenem resistant Acinetobacter baumannii isolates from hospital environment[J]. Microb Pathog, 2016, 99:204-208.
[9] Vatan A, Saltoglu N, Yemisen M, et al. Association between biofilm and multi/extensive drug resistance in diabetic foot infection[J]. Int J Clin Pract, 2018, 72(3):e13060.
[10] Gurung J, Khyriem AB, Banik A, et al. Association of biofilm production with multidrug resistance among clinical isolates of Acinetobacter baumannii and Pseudomonas aeruginosa from intensive care unit[J]. Indian J Crit Care Med, 2013, 17(4):214-218.
[11] 赖其伟, 黄丽芳. 不鲍曼不动杆菌生物被膜与耐药性的相关性分析[J]. 国际检验医学杂志, 2016, 37(2):153-154.
[12] Gopal R, Kim YG, Lee JH, et al. Synergistic effects and antibiofilm properties of chimeric peptides against multidrug-resistant Acinetobacter baumannii strains[J]. Antimicrob Agents Chemother, 2014, 58(3):1622-1629.
[13] Qi L, Li H, Zhang C, et al. Relationship between Antibiotic Resistance, Biofilm Formation, and Biofilm-Specific Resistance in Acinetobacter baumannii[J]. Front Micro-biol, 2016, 7:483.
[14] 刘原, 柯蕊, 杨妮, 等. 呼吸道分离鲍曼不动杆菌生物被膜形成能力与耐药性关系的研究[J]. 中国实验诊断学, 2015, 19(8):1243-1246.
[15] Wen H, Wang K, Liu Y, et al. Population dynamics of an Acinetobacter baumannii clonal complex during colonization of patients[J]. J Clin Microbiol, 2014, 52(9):3200-3208.
[16] Abdi-Ali A, Hendiani S, Mohammadi P, et al. Assessment of Biofilm Formation and Resistance to Imipenem and Ciprofloxacin among Clinical Isolates of Acinetobacter baumannii in Tehran[J]. Jundishapur J Microbiol, 2014, 7(1):e8606.
[17] Tilley D, Law R, Warren S, et al. CpaA a novel protease from Acinetobacter baumannii clinical isolates deregulates blood coagulation[J]. FEMS Microbiol Lett, 2014, 356(1):53-61.
[18] Ghasemi E, Ghalavand Z, Goudarzi H, et al. Phenotypic and Genotypic Investigation of Biofilm Formation in Clinical and Environmental Isolates of Acinetobacter baumannii[J]. Arch Clin Infect Dis, 2018, 13(4):e12914.
[19] Thummeepak R, Kongthai P, Leungtongkam U, et al. Distribution of virulence genes involved in biofilm formation in multi-drug resistant Acinetobacter baumannii clini-cal isolates[J]. Int Microbiol, 2016, 19(2):121-129.
[20] Fallah A, Rezaee MA, Hasani A, et al. Frequency of bap and cpaA virulence genes in drug resistant clinical isolates of Acinetobacter baumannii and their role in biofilm formation[J]. Iran J Basic Med Sci, 2017, 20(8):849-855.
[21] Ali HM, Salem MZM, El-Shikh MS, et al. Investigation of the Virulence Factors and Molecular Characterization of the Clonal Relations of Multidrug-Resistant Acinetobacter baumannii Isolates[J]. J AOAC Int, 2017, 100(1):152-158.
[22] Liu H, Wu YQ, Chen LP, et al. Biofilm-Related Genes: Analyses in Multi-Antibiotic Resistant Acinetobacter Baumannii Isolates From Mainland China[J]. Med Sci Monit, 2016, 22:1801-1807.
[23] 童飞, 汪跃国, 范骁钦. 2015-2016年重症监护病房鲍曼不动杆菌感染及耐药分析[J]. 安徽医学, 2017, 38(12):1583-1585.
[24] Selasi GN, Nicholas A, Jeon H, et al. Differences in Biofilm Mass, Expression of Biofilm-Associated Genes, and Resistance to Desiccation between Epidemic and Sporadic Clones of Carbapenem-Resistant Acinetobacter baumannii Sequence Type 191[J]. PLoS One, 2016, 11(9):e0162576.
[25] Marti S, Nait Chabane Y, Alexandre S, et al. Growth of Acinetobacter baumannii in pellicle enhanced the expression of potential virulence factors[J]. PLoS One, 2011, 6(10):e26030.
Options
Outlines

/

〈 〉