Objective: To investigate the diagnostic value and clinical significance of anti-zymogen granule membrane glycoprotein 2 antibody（GP2） and anti-CUB and zonapellucida-like domains-containing protein 1 antibody（CUZD1） in patients with Crohn's disease (CD）. Methods: A total of 128 CD patients, 82 ulcerative colitis (UC) patients and 60 healthy controls were enrolled. Serum anti-GP2 and anti-CUZD1 antibodies were detected by indirect immunofluorescence assay (IIF). Receiver operator characteristic curve (ROC) was used to evaluate the diagnostic efficiency of anti-GP2 and anti-CUZD1 antibodies in single and combining form. The correlation between the two antibodies and clinical characteristics were analyzed by Logistic regression. Results: ① The positive rates of serum anti-GP2 antibodies in CD, UC and health control groups were 45.3%, 11.0%, 0%, respectively. There was significant difference between CD and UC groups（ χ2=27.12, P<0.01）and between CD and health control groups （ χ2=39.32, P<0.01）. Positive rates of serum anti-CUZD1 antibodies in CD, UC and health control groups were 19.5%, 14.6%, 0%, respectively. There was significant difference between CD and health control groups（ χ2=13.52, P<0.01）, but no significant differences was found between CD and UC groups （ χ2=0.83, P=0.36）. ② The sensitivity of anti-GP2 antibodies was 45.3%, the specificity was 93.7% and the area under ROC curve (AUC）was 0.695; the sensitivity of anti-CUZD1 antibodies was 19.5%, the specificity was 91.5% and AUC was 0.555. The diagnostic value of combined anti-GP2 and anti-CUZD1 antibodies was significantly higher than one of them only (Z=4.44, 2.57, respectively, all P<0.01）. ③ Multiple linear regression analysis indicated a positive correlation between anti-GP2 antibody and the perianal, penetrated and ileocolon form of CD（P<0.05）; and anti-CUZD1 antibody was associated with stenosis and terminal ileum form of CD（P<0.05）. Conclusions: Anti-GP2 and anti-CUZD1 antibodies are potential biomarker for the diagnosis of CD. The combination of anti-GP2 and anti-CUZD1 antibodies have higher diagnostic value than one of them only, and could be used to stratify CD patients phenotypically for providing references to establish specific therapeutic strategies.
ZENG Junxiang, LUO Ting, GE Wensong, PAN Xiujun, SHEN Lisong
. Diagnostic value and clinical significance of anti-GP2 and anti-CUZD1 antibodies in Crohn's disease[J]. Journal of Diagnostics Concepts & Practice, 2018
, 17(04)
: 433
-438
.
DOI: 10.16150/j.1671-2870.2018.04.015
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