Objective: To investigate the effect of antimicrobial stewardship（AMS） on enhancing submission rate of microbiological specimens before therapeutic use of antimicrobial agents. Methods：January 2017 to June 2017 was the pre-intervention group; July 2017 to September 2017 was the post-intervention group. Submission rate of microbiological specimens before therapeutic use of antimicrobial agents were compared between the two groups. Constituent ratio of different microbiological specimens was also compared between the two groups. Results: The submission rates of microbiolo-gical specimens for general, restricted and special therapeutic use of antimicrobial agents before and after AMS increased from 49.46%, 47.82%, 69.91% to 61.94%, 62.11%, 83.45%, respectively (P all <0.01). The submission rate of microbiological specimens for general and special therapeutic use of antimicrobial agents before and after AMS in key departments reached the assessment criteria.The constituent ratio of sputum culture specimen was decreased from 53.46% to 51.66%( P<0.01), and the constituent ratio of blood culture and aseptic humoral specimen increased from 11.96% and 3.60% to 15.32% and 4.10%(P<0.01, P<0.05). Conclusions: AMS can effectively improve the submission rate of microbiological specimens before therapeutic use of antimicrobial agents and the quality of specimens for inspection; it provides laboratory evidence for the rational use of antibiotics.