Original articles

Evaluation of TDR NH-AST kit for testing antimicrobial susceptibility of Haemophilus influenza

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  • Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Received date: 2019-08-14

  Online published: 2019-10-25

Abstract

Objective: Haemophilus influenzae is a pathogen that can cause severe infections. Accurate determination of H. influenzae susceptibility is important to ensure treatment efficiency. This study aimed to evaluatethe performance of TDR NH-AST kit for testing the antimicrobial susceptibility of Haemophilus influenzae. Methods: A total of 101 isolates were collected from clinical laboratories. Broth microdilution method was performed following the Clinical and Laboratory Standards Institute guideline M07-A10 as the reference method. The category agreement (CA), essential agreement (EA), major discrepancy (MD) and very major discrepancy (VDM) were used to evaluate the agreement between the TDR NH-AST kit and reference method according to ISO 20776-2. Kappa value was calculated and McNemar test was performed to analyze the consistency between the two methods. Results: A total of 18 antimicrobial drugs were involved. Apart from meropenem, the Kappa values of the other antimicrobial drug susceptibility test were all higher than 0.75, and the P va-lues were all higher than 0.05 except ampicillin. The overall values of CA, EA, MD and VMD were 97.80%, 95.38%, 2.07% and 3.29%, respectively. Although EAs of meropenem, azithromycin and ciprofloxacin were slightly less than 90% (88.12%, 84.16% and 88.12%, respectively), all the other antibacterial drugs were more than 90%, and CAs of all antibacterial drugs were more than 90%. Conclusions: TDR NH-AST has a good consistency, accuracy and reliability in the detection of antimicrobial susceptibility of H. influenzae, and could meet the requirements of routine clinical antimicrobial susceptibility test of H. influenzae.

Cite this article

LI Xinxin, XIAO Shuzhen, TAN Xijing, NI Yuxing, HAN Lizhong . Evaluation of TDR NH-AST kit for testing antimicrobial susceptibility of Haemophilus influenza[J]. Journal of Diagnostics Concepts & Practice, 2019 , 18(05) : 526 -531 . DOI: 10.16150/j.1671-2870.2019.05.008

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