Original article

An investigation on pathogenicity and drug sensitivity of 1 097 isolates from patients with fungal vaginitis in Jiading District of Shanghai

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  • 1. Department of Clinical Laboratory, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;
    2. Department of Clinical Laboratory, Shanghai Jiading District Central Hospital, Shanghai 201800, China;
    3. Department of Clinical Laboratory, Maternal and Child Health Hospital of Jiading District, Shanghai 201800, China;
    4. Department of Clinical Laboratory, Jiading District Hospital of Traditional Chinese Medicine, Shanghai 201800, China;
    5. Department of Clinical Laboratory, Nanxiang Hospital, Jiading District, Shanghai 201802, China;
    6. Department of Clinical Laboratory, Jiading District Anting Hospital, Shanghai 201805, China

Received date: 2017-03-30

  Online published: 2017-08-25

Abstract

Objective: To investigate the distribution and drug sensitivity of pathogens of fungal vaginitis in Jiading District of Shanghai for providing a guidance for clinical diagnosis and treatment of the disease. Methods: Altogether 1 097 leucorrhea specimens were collected from patients with fungal vaginitis in obstetrics and gynecology department from 5 hospitals(Jiading District Central Hospital, Maternal and Child Health Hospital, Jiading District Hospital of Traditional Chinese Medicine, Nanxiang Hospital and Anting Hospital), from July 2015 to June 2016. The specimens were cultured and drug sensitivity test was performed. The results were analyzed by SPSS 13.0. Results: There was no significant difference in distribution of pathogens among these hospitals, and Candida albicans was the most common (81.5%). The highest proportion (14.6 percent) of non-Candidaalbicans was found in Candidaglabrata. The proportion of Candida albicans in recurrent patients was 67.0%. Overall sensitive rates of all strains to fluconazole, voriconazole and itraconazole were 98.9%, 92.0% and 70.0%, respectively. The sensitivity of Candida albicans and non-Candida albicans to 3 azoles were 99.6%, 92.6%, 72.4% and 84.8%, 89.2% and 59.1%, respectively. For hospital, the five hospitals showed significant differences in sensitivity of drugs in the case of voriconazole and itraconazole. Conclusions: Candida albicans is the main pathogen of fungal vaginitis in Jiading District of Shanghai. The distribution of pathogens in patients with recurrent fungal vaginitis shows a trend of diversity. Some pathogens appeare with more obvious itraconazole resistance. There are also differences in drug sensitivity among the strains and different hospitals. To emphasize the pathogen examination and drug sensitivity test of fungal vaginitis is helpful for clinicians to prescribe better clinical treatment.

Cite this article

XU Weixin, CAI Xushan, HUANG Zhongfa, HUANG Qiulan, HE Yanhong, XIANG Mingjie . An investigation on pathogenicity and drug sensitivity of 1 097 isolates from patients with fungal vaginitis in Jiading District of Shanghai[J]. Journal of Diagnostics Concepts & Practice, 2017 , 16(04) : 409 -413 . DOI: 10.16150/j.1671-2870.2017.04.013

References

[1] 彭文清, 谢志强. 假丝酵母菌性阴道炎的菌种分类及耐药性监测[J]. 中华医院感染学杂志,2013,23(6):1467-1469.
[2] Bassetti M, Ansaldi F, Nicolini L,et al.Incidence of candidaemia and relationship with fluconazole use in an intensive care unit[J]. J Antimicrob Chemother,2009,64(3):625-629.
[3] Lass-Flörl C.The changing face of epidemiology of invasive fungal disease in Europe[J]. Mycoses,2009,52(3):197-205.
[4] Clinical Laboratory Standards Institute.Reference Method for Broth Dilution Antifungal susceptibility testing of yeast[M]. Clinical Laboratory Standards Institute,2012,32:1-23.
[5] 张文, 柏彩英, 周强, 等. 念珠菌性阴道炎的菌种分布及耐药性分析[J]. 皮肤性病诊疗学杂志,2012,19(3):139-141.
[6] 张启翔,余军平. 245例女性阴道假丝酵母菌病的真菌分布及耐药性分析[J]. 中华医院感染学杂志,2012,22(6):1306-1307.
[7] 雷云. 复发性外阴阴道假丝酵母菌病致病菌的药敏及其基因分布[J]. 广西医学,2014,28(3):288-290.
[8] 赵辉, 王群, 赵冰, 等. 真菌性阴道炎假丝酵母菌属临床感染及药敏结果分析[J]. 中国现代医生,2014,52(13):78-79.
[9] Liu Q, Luyten W, Pellens K, et al.Antifungal activity in plants from Chinese traditional and folk medicine[J]. J Ethnopharmacol,2012,143(3):772-778.
[10] Khajeh E, Hosseini Shokouh SJ, Rajabibazl M, et al.Antifungal effect of Echinophora platyloba on expression of CDR1 and CDR2 genes in fluconazole-resistant Candida albicans[J]. Br J Biomed Sci,2016,73(1):44-48.
[11] Brandolt TM, Klafke GB, Gonçalves CV, et al.Prevalence of Candida spp. in cervical-vaginal samples and the in vitro susceptibility of isolates[J]. Braz J Microbiol,2017, 48(1):145-150.
[12] 蒙在杨, 秦耀春, 潘秀贤. 344对性伴侣生殖器念珠菌感染的菌型分析[J]. 中国真菌学杂志,2010,5(3):168-169.
[13] 金丽, 孙曼, 张昭, 等. 血清G 试验在深部真菌感染中的诊断价值[J]. 安徽医学,2016,37(5):587-589.
[14] 杨柳, 李鹏宇, 王智. 真菌性阴道炎患者的假丝酵母菌感染及耐药性研究[J]. 检验医学与临床,2015,12(12):1730-1732.
[15] 郭玉荣, 赵晓丽. 复发性外阴阴道假丝酵母菌病的病原菌鉴定与药敏分析[J]. 国际检验医学杂志,2016,37(2):251-253.
[16] 邹绣, 应颖, 胡雪飞, 等. 念珠菌性阴道炎的菌种鉴定及药敏分析[J]. 南昌大学学报: 医学版,2013,53(3):22-24.
[17] 章强强. 我国真菌感染的实验室检测现状[J]. 诊断学理论与实践,2016,15(1):1-4.
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