Objective: To investigate correlation of human oocyte spindle and its retardant value with the outcome of embryo development after ICSI using mini stimulation protocol. Methods: A total of 354 patients placed on ICSI cycles were enrolled from December 2017 through June 2019, and the relevant clinical and laboratory data were retrieved and retrospectively analyzed. The rates of normal fertilization, cleavage, quality embryo, blastocyst formation and pregnancy were compared between individuals with and without visible spindle (SP⁺, SP^- group). In SP⁺ group, The retardant value was calculated and compared between patients with normal and abnormal fertilization; cleavage and non-cleavage; quality and non-quality embryo; blastocyst formation and non-blastocyst formation; clinical pregnancy and non-clinical pregnancy. Results: The rates of normal fertilization, cleavage, quality embryo, blastocyst formation and clinical pregnancy in patients of SP⁺ group were significantly higher than those in SP^- group （83.72% vs 72.22%, 98.14% vs 85.16%, 73.07% vs 62.50%, 50.67% vs 36.76%, 45.56% vs 27.27%, P<0.05）, while the rate of abnormal fertilization in SP⁺ group was lower（4.26% vs 16.11%, P<0.0001）. The spindle retardant value in patients with normal fertilization, cleavage, quality embryo and pregnancy were significantly higher than those without （2.01 vs 1.81, 2.03 vs 1.66, 2.03 vs 1.91, 2.20 vs 2.02、2.19 vs 2.01, P<0.05), however, there was no significant difference in spindle volume, area and spindle angle with the first polar body. With the increase of age in patients, the number of SP⁺ eggs obtained per patient decreased[<35 years old, 3.30; 35-40 years old, 2.91; ≥40 years old, 2.46] (P=0.025). Conclusion: ICSI using oocytes with visible spindle may enable higher rate of normal fertilization, cleavage, blastocyst formation and lower rate of abnormal fertilization. The embryos developed from oocytes with higher spindle retardant value could improve the clinical pregnancy rate.