Correlation of spindle parameters of human mature oocytes with the outcomes of ICSI performed with mini-stimulation protocol

doi:10.16150/j.1671-2870.2020.02.012

Abstract

Abstract:

Objective: To investigate correlation of human oocyte spindle and its retardant value with the outcome of embryo development after ICSI using mini stimulation protocol. Methods: A total of 354 patients placed on ICSI cycles were enrolled from December 2017 through June 2019, and the relevant clinical and laboratory data were retrieved and retrospectively analyzed. The rates of normal fertilization, cleavage, quality embryo, blastocyst formation and pregnancy were compared between individuals with and without visible spindle (SP⁺, SP^- group). In SP⁺ group, The retardant value was calculated and compared between patients with normal and abnormal fertilization; cleavage and non-cleavage; quality and non-quality embryo; blastocyst formation and non-blastocyst formation; clinical pregnancy and non-clinical pregnancy. Results: The rates of normal fertilization, cleavage, quality embryo, blastocyst formation and clinical pregnancy in patients of SP⁺ group were significantly higher than those in SP^- group （83.72% vs 72.22%, 98.14% vs 85.16%, 73.07% vs 62.50%, 50.67% vs 36.76%, 45.56% vs 27.27%, P<0.05）, while the rate of abnormal fertilization in SP⁺ group was lower（4.26% vs 16.11%, P<0.0001）. The spindle retardant value in patients with normal fertilization, cleavage, quality embryo and pregnancy were significantly higher than those without （2.01 vs 1.81, 2.03 vs 1.66, 2.03 vs 1.91, 2.20 vs 2.02、2.19 vs 2.01, P<0.05), however, there was no significant difference in spindle volume, area and spindle angle with the first polar body. With the increase of age in patients, the number of SP⁺ eggs obtained per patient decreased[<35 years old, 3.30; 35-40 years old, 2.91; ≥40 years old, 2.46] (P=0.025). Conclusion: ICSI using oocytes with visible spindle may enable higher rate of normal fertilization, cleavage, blastocyst formation and lower rate of abnormal fertilization. The embryos developed from oocytes with higher spindle retardant value could improve the clinical pregnancy rate.

Key words: Mature oocytes, Spindle, Retardant value, Embryo development

CLC Number:

R715.9

HUANG Ya, WU Yunlong, XU Li, GE Fengju, CHEN Yaning, QI Xiujuan. Correlation of spindle parameters of human mature oocytes with the outcomes of ICSI performed with mini-stimulation protocol[J]. Journal of Diagnostics Concepts & Practice, 2020, 19(02): 162-167.

Figures/Tables 5

References 14

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胚胎级别	受精第2天			受精第3天
胚胎级别	细胞数	卵裂球大小	碎片	细胞数	卵裂球大小	碎片
Ⅰ	3~5	均匀	<5%	6~10	均匀	<5%
Ⅱ	3~5	均匀	5%~15%	6~10	均匀	5%~15%
	2	均匀	<5%	4~5	均匀	<5%
Ⅲ	3~5	不均匀	15%~25%	6~10	不均匀	15%~25%
	2	均匀	10%~25%	4~6	均匀	10%~25%
Ⅳ	任何细胞数,碎片25%~50%			任何细胞数,碎片25%~50%
Ⅴ	任何细胞数,碎片>50%			任何细胞数,碎片>50%

年龄组（岁）	患者数（%）	平均年龄（$\bar{x}\pm s$）	基础FSH （U/L）	基础LH （U/L）	基础E₂ （pg/L）	AMH （ng/mL）	MⅡ卵数（个）	人均获卵数（个）	SP⁺卵数（个）	人均获SP⁺ 卵数（个）
<35	103（29.10）	31.0±2.6	12.28±8.35	4.03±2.41	28.73±14.14^d）	2.09±2.02	350	3.44±2.53	295	3.30±2.22^d）
35~40	146（41.20）	37.4±1.7^a）	12.22±1.66	4.17±2.75	32.29±15.61	1.34±1.66^a）	440	3.05±2.22	367	2.91±2.10
≥40	105（29.70）	43.5±1.9^a）,b)	14.99±12.21^c)	4.77±4.16	35.77±18.83	0.78±0.87^a）,b)	305	2.85±2.51	253	2.46±1.99
P值	-	<0.001	0.037	0.198	0.011	<0.001	-	0.189	-	0.025

分组	正常受精率（%）	异常受精率（%）	卵裂率（%）	优胚率（%）	囊胚形成率（%）	临床妊娠率（%）
SP⁺	766/915（83.72）	39/915（4.26）	790/805（98.14）	551/754（73.07）	152/300（50.67）	41/90（45.56）
SP^-	130/180（72.22）	29/180（16.11）	132/155（85.16）	80/128（62.50）	25/68（36.76）	6/22（27.27）
P	0.000 3	<0.000 1	<0.000 1	0.014 2	0.038 3	0.013 8

组别	体积(μm³)	面积(μm²)	角度(°)^a)	阻滞值(nm)
正常受精组	241.30±115.99	84.80±26.75	16.18±20.31	2.01±0.56
异常受精组	243.62±127.34	85.16±29.47	18.19±19.76	1.81±0.51
P值	0.904	0.935	0.906	0.027
卵裂组	241.49±115.38	84.56±26.78	16.25±20.31	2.03±0.57
未卵裂组	190.42±83.19	71.95±19.59	5.88±9.32	1.66±0.27
P值	0.127	0.098	0.078	0.028
优胚组	241.65±114.58	85.09±26.53	16.77±21.03	2.03±0.52
非优胚组	241.13±117.56	83.93±26.99	14.98±18.44	1.91±0.52
P值	0.954	0.581	0.260	0.003
囊胚形成组	249.97±120.66	85.15±26.01	13.88±16.74	2.20±0.61
囊胚未形成组	249.07±116.08	86.10±26.40	15.85±18.24	2.02±0.65
P值	0.952	0.773	0.375	0.029
临床妊娠组	247.97±118.66	84.82±24.74	14.98±15.74	2.19±0.53
临床未妊娠组	245.99±115.69	83.92±23.81	13.85±16.24	2.01±0.56
P值	0.932	0.918	0.802	0.028

年龄分组（岁）	SP⁺卵数	阻滞值（nm）	F值	P值
<35	295	2.1±0.6
35~40	367	2.0±0.6	6.796	0.001
≥40	253	1.9±0.5