Objective: To compare the performance of BioIC Allergen specific IgE Detection Kit (micro-fluid chip enzyme-linked fluoroimmunoassay, ELFA) and EUROLINE Atopy China (immunoblotting test, IBT) for detecting allergen-specific IgE. Methods: The stability and dilution of these two methods were compared by using mixed human plasma with strong positivity for multi-sIgE and horse plasma, and the positive levels of common allergens were also compared. Results: The CV% of ELFA was obviously lower than that of IBT in six kinds of allergens including mite mix, crab, shrimp, peanut, egg white and dog dander(T=6.20, P<0.01), while was slight higher in cat dander,cockroach and milk(T=2.43, P>0.05). Both IBT and ELFA showed a positive correlation of testing result with concentration dilution. There was a high consistency of positive grading for mite mix and cockroach, while a weak consistency for peanuts and other five kinds of allergens. Conclusions: There are some differences between ELFA and IBT, but both could reflect the concentration of sIgE. Compared with IBT, ELFA has a higher stability and sensitivity, covering more allergens, less testing time and sample volume. ELFA could be used in allergen-specific IgE testing in clinical laboratory.