Objective: To investigate the expression of FENDRR, a long non-coding RNA gene, in colorectal cancer tissue and to detect the correlation of FENDRR expression with prognosis of colorectal cancer, and to explore the correlation between FENDRR and E-cadherin as well as N-cadherin. Methods: A total of 90 pairs of colorectal cancer tissue and matched para-carcinoma tissue samples were collected.The expression of FENDRR was detected by real time fluorescencequantitative polymerase chain reaction(rtfq-PCR). Western blotting analysis was used to detect the expression of E-cadherin and N-cadherin protein in colorectal cancer,and their correlations with FENDRR were analyzed. Results: The expression of FENDRR was decreased in cancer tissue (8.29±0.13) in relative to their corresponding para-cancer tissue(8.59±0.18), and the difference was statistically significant (t=13.666, P<0.01). E-cadherin was negatively correlated with N-cadherinin colorectal cancer tissue r=-0.584, P<0.01）; FENDRR expression was positively correlated with E-cadherin(r=0.778, P<0.01) and negatively correlated with N-cadherin protein (r=-0.692, P<0.01). The overall survival time of colorectal cancer patients with high FENDRR expression was higher than that of patients with low FENDRR expression (90.0 months vs 49.5 months, P=0.041). Conclusions: FENDRR shows an inhibiting effect on development of colorectal cancer, and has certain relationship with E-cadherin and N-cadherin.FENDRR might exert its inhibitory effect on metastasis by influencing the epithelial-mesenchymal transition(EMT) process. Detection of FENDRR might be useful for predicting the prognosis of colorectal cancer.
CHEN Ying, HE Lili, JIANG Feng
. Expression of long non-coding RNA FENDRR in human colorectal cancer and its correlation with prognosis[J]. Journal of Diagnostics Concepts & Practice, 2018
, 17(01)
: 87
-91
.
DOI: 10.16150/j.1671-2870.2018.01.016
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