Objective: To construct a training program on emergency response ability for all staff in emergency medical wards under COVID-19 epidemic, and to evaluate its clinical effect. Methods: A targeted training program for emergency response ability of all staff in the emergency medical ward was established through literature review, brain storming and online conference discussion. The scheme includes seven modules: training system, training plan, training outline, training course, teaching team, training materials and teaching evaluation. For medical staff, other staff (nursing workers, work attendance, cleaning), patients and family members of different categories of personnel, advanced version, basic version and popular science version are designed respectively, and the training content closely fitted the needs of epidemic prevention and skills required in different groups. During the period from April 5 to May 10, 2022, the training program was conducted to train the emergency response ability in all staff,and effect was evaluated using questionnaires invstigation before and after training, oral questions, operation assessment and on-site inspection, and the incidence of clinical actual infection was monitered. Results: The score after traning on response ability to infectious disease emergencies in medical staff( 161.27±14.13) was higher than before the training (143.38±23.10) (P<0.05). After the training, the qualified rate of "hand hygiene" and "wearing and taking off isolation clothes" in nursing workers, cleaning workers and work attendance increased from 62.5% (before the training) to 100.0%, and the qualified rate of oral question answering increased from 50.0% (before the training) to 87.5%. The correct wearing rate of masks for patients and their families increased from 66.1% to 89.3%, and the standard rate of hand hygiene increased from 75.0% to 92.0%. The score on satisfaction was (16.36±2.03), which indicates that all staff had a good experience of participating in the training process. There were no case of novel coronavirus infection. Conclusions: Under the background of COVID-19 epidemic, the whole staff-emergency capacity training program established in this study is reasonable and feasible, which can effectively improve the emergency capacity of personnel, urge everyone to participate in nosocomial infection prevention and control, and ensure the normal epidemic control in the ward.