Journal of Diagnostics Concepts & Practice >

2020 , Vol. 19 >Issue 05: 510 - 515

DOI: https://doi.org/10.16150/j.1671-2870.2020.05.012

Original articles

Expression of microRNA-337-3p in oral squamous cell carcinoma and its clinical significance

Expand
  • Department of Clinical Laboratory, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

Received date: 2020-03-23

  Online published: 2022-07-14

Fold

Abstract

Objective: To investigate the expression of microRNA (miRNA)-337-3p in oral squamous cell carcinoma (OSCC) and its clinical significance. Methods: The expression of miRNA-337-3p in OSCC cancer and paracancerous tissue of 20 OSCC patients was determined, and the correlation of miRNA-337-3p expression with clinical-pathological parameter was analyzed. Scratch test was performed to detect the effect of miRNA-337-3p on migration ability of OSCC cell line HN30 cells, and the possible molecular mechanism was explored. Results: Expression of miRNA-337-3p in OSCC was significantly lower than that in paracancerous tissue(P<0.000 1), and was correlated significantly with the status of lymph node metastasis (P<0.05). Patients with lymph node metastasis (N1+N2) had a lower expression level of miRNA-337-3p than those without lymph node metastasis (N0). Overexpression of miRNA-337-3p in HN30 cells inhibited cell migration, whereas suppression of miRNA-337-3p led to increase of cell migration. miRNA-337-3p could inhibit the expression of matrix metalloproteinase-14 (MMP-14). Conclusions: miRNA-337-3p plays a suppressive role in OSCC, inhibitingthe invasion and metastasis of OSCC cells, and is expected to be used as a biomarker for assessing the invasion and metastasis of OSCC and helping the monitoring of prognosis.

Key words： Oral squamous cell carcinoma; microRNA; microRNA-337-3p; Metastasis

Cite this article

ZHANG Xingming, GU Wenli . Expression of microRNA-337-3p in oral squamous cell carcinoma and its clinical significance[J]. Journal of Diagnostics Concepts & Practice, 2020 , 19(05) : 510 -515 . DOI: 10.16150/j.1671-2870.2020.05.012

References

[1] 何保昌, 高小叶, 陈法, 等. 口腔癌发病影响因素病例对照研究[J]. 中国公共卫生, 2014, 30(2):248-250.
[2] Gao W, Li JZ, Chen SQ, et al. Decreased brain-expressed X-linked 4 (BEX4) expression promotes growth of oral squamous cell carcinoma[J]. J Exp Clin Cancer Res, 2016, 35(1):92.
[3] Miller KD, Siegel RL, Lin CC, et al. Cancer treatment and survivorship statistics, 2016[J]. CA Cancer J Clin, 2016, 66(4):271-289.
[4] Malik UU, Zarina S, Pennington SR. Oral squamous cell carcinoma: key clinical questions, biomarker discovery, and the role of proteomics[J]. Arch Oral Biol, 2016, 63:53-65.
[5] Mello FW, Melo G, Pasetto JJ, et al. The synergistic effect of tobacco and alcohol consumption on oral squamous cell carcinoma: a systematic review and meta-ana-lysis[J]. Clin Oral Investig, 2019, 23(7):2849-2859.
[6] Li WC, Lee PL, Chou IC, et al. Molecular and cellular cues of diet-associated oral carcinogenesis--with an emphasis on areca-nut-induced oral cancer development[J]. J Oral Pathol Med, 2015, 44(3):167-177.
[7] Zhang J, Raju GS, Chang DW, et al. Global and targeted circulating microRNA profiling of colorectal adenoma and colorectal cancer[J]. Cancer, 2018, 124(4):785-796.
[8] Kim MH, Cho JS, Kim Y, et al. Discriminating between terminal- and non-terminal respiratory unit-type lung adenocarcinoma based on microRNA profiles[J]. PLoS One, 2016, 11(8):e0160996.
[9] Liang Z, Kong R, He Z, et al. High expression of miR-493-5p positively correlates with clinical prognosis of non small cell lung cancer by targeting oncogene ITGB1[J]. Oncotarget, 2017, 8(29):47389-47399.
[10] Cinegaglia NC, Andrade SC, Tokar T, et al. Integrative transcriptome analysis identifies deregulated microRNA-transcription factor networks in lung adenocarcinoma[J]. Oncotarget, 2016, 7(20):28920-28934.
[11] Feng H, Ge F, Du L, et al. MiR-34b-3p represses cell proliferation, cell cycle progression and cell apoptosis in non-small-cell lung cancer (NSCLC) by targeting CDK4[J]. J Cell Mol Med, 2019, 23(8):5282-5291.
[12] Swellam M, Zahran RFK, Ghonem SA, et al. Serum miRNA-27a as potential diagnostic nucleic marker for breast cancer[J]. Arch Physiol Biochem, 2019,1-7.
[13] Wang Z, Wang J, Yang Y, et al. Loss of has-miR-337-3p expression is associated with lymph node metastasis of human gastric cancer[J]. J Exp Clin Cancer Res, 2013, 32(1):76.
[14] Kim SY, Lee YH, Bae YS. MiR-186, miR-216b, miR-337-3p, and miR-760 cooperatively induce cellular senescence by targeting α subunit of protein kinase CKⅡ in human colorectal cancer cells[J]. Biochem Biophys Res Commun, 2012, 429(3-4):173-179.
[15] Xiang X, Mei H, Zhao X, et al. miRNA-337-3p suppresses neuroblastoma progression by repressing the transcription of matrix metalloproteinase 14[J]. Oncotarget, 2015, 6(26):22452-22466.
[16] Nagase H, Visse R, Murphy G. Structure and function of matrix metalloproteinases and TIMPs[J]. Cardiovasc Res, 2006, 69(3):562-573.
[17] Conlon GA, Murray GI. Recent advances in understan-ding the roles of matrix metalloproteinases in tumour inva-sion and metastasis[J]. J Pathol, 2019, 247(5):629-640.
[18] Dufour A, Sampson NS, Zucker S, et al. Role of the hemopexin domain of matrix metalloproteinases in cell migration[J]. J Cell Physiol, 2008, 217(3):643-651.
[19] Remacle AG, Cieplak P, Nam DH, et al. Selective function-blocking monoclonal human antibody highlights the important role of membrane type-1 matrix metalloproteinase (MT1-MMP) in metastasis[J]. Oncotarget, 2017, 8(2):2781-2799.
[20] Li Y, Kuscu C, Banach A, et al. miR-181a-5p inhibits cancer cell migration and angiogenesis via downregulation of matrix metalloproteinase-14[J]. Cancer Res, 2015, 75(13):2674-2685.
[21] Momen-Heravi F, Bala S. Emerging role of non-coding RNA in oral cancer[J]. Cell Signal, 2018, 42:134-143.
[22] Huang SH, O'Sullivan B. Overview of the 8th edition TNM classification for head and neck cancer[J]. Curr Treat Options Oncol, 2017, 18(7):40.
[23] Zhuang Q, Shen J, Chen Z, et al. MiR-337-3p suppresses the proliferation and metastasis of clear cell renal cell carcinoma cells via modulating Capn4[J]. Cancer Biomark, 2018, 23(4):515-525.
[24] Cao XM. Role of miR-337-3p and its target Rap1A in modulating proliferation, invasion, migration and apoptosis of cervical cancer cells[J]. Cancer Biomark, 2019, 24(3):257-267.
[25] Yuan H, Wei R, Xiao Y, et al. RHBDF1 regulates APC-mediated stimulation of the epithelial-to-mesenchymal transition and proliferation of colorectal cancer cells in part via the Wnt/β-catenin signalling pathway[J]. Exp Cell Res, 2018, 368(1):24-36.
Options
Outlines

/

〈 〉