Expression of microRNA-337-3p in oral squamous cell carcinoma and its clinical significance

doi:10.16150/j.1671-2870.2020.05.012

Abstract

Abstract:

Objective: To investigate the expression of microRNA (miRNA)-337-3p in oral squamous cell carcinoma (OSCC) and its clinical significance. Methods: The expression of miRNA-337-3p in OSCC cancer and paracancerous tissue of 20 OSCC patients was determined, and the correlation of miRNA-337-3p expression with clinical-pathological parameter was analyzed. Scratch test was performed to detect the effect of miRNA-337-3p on migration ability of OSCC cell line HN30 cells, and the possible molecular mechanism was explored. Results: Expression of miRNA-337-3p in OSCC was significantly lower than that in paracancerous tissue(P<0.000 1), and was correlated significantly with the status of lymph node metastasis (P<0.05). Patients with lymph node metastasis (N₁+N₂) had a lower expression level of miRNA-337-3p than those without lymph node metastasis (N₀). Overexpression of miRNA-337-3p in HN30 cells inhibited cell migration, whereas suppression of miRNA-337-3p led to increase of cell migration. miRNA-337-3p could inhibit the expression of matrix metalloproteinase-14 (MMP-14). Conclusions: miRNA-337-3p plays a suppressive role in OSCC, inhibitingthe invasion and metastasis of OSCC cells, and is expected to be used as a biomarker for assessing the invasion and metastasis of OSCC and helping the monitoring of prognosis.

Key words: Oral squamous cell carcinoma, microRNA, microRNA-337-3p, Metastasis

CLC Number:

R730.261

ZHANG Xingming, GU Wenli. Expression of microRNA-337-3p in oral squamous cell carcinoma and its clinical significance[J]. Journal of Diagnostics Concepts & Practice, 2020, 19(05): 510-515.

Figures/Tables 6

References 25

[1]	何保昌, 高小叶, 陈法, 等. 口腔癌发病影响因素病例对照研究[J]. 中国公共卫生, 2014, 30(2):248-250.
[2]	Gao W, Li JZ, Chen SQ, et al. Decreased brain-expressed X-linked 4 (BEX4) expression promotes growth of oral squamous cell carcinoma[J]. J Exp Clin Cancer Res, 2016, 35(1):92. doi: 10.1186/s13046-016-0355-6 URL
[3]	Miller KD, Siegel RL, Lin CC, et al. Cancer treatment and survivorship statistics, 2016[J]. CA Cancer J Clin, 2016, 66(4):271-289. doi: 10.3322/caac.21349 URL
[4]	Malik UU, Zarina S, Pennington SR. Oral squamous cell carcinoma: key clinical questions, biomarker discovery, and the role of proteomics[J]. Arch Oral Biol, 2016, 63:53-65. doi: 10.1016/j.archoralbio.2015.11.017 URL
[5]	Mello FW, Melo G, Pasetto JJ, et al. The synergistic effect of tobacco and alcohol consumption on oral squamous cell carcinoma: a systematic review and meta-ana-lysis[J]. Clin Oral Investig, 2019, 23(7):2849-2859. doi: 10.1007/s00784-019-02958-1 URL
[6]	Li WC, Lee PL, Chou IC, et al. Molecular and cellular cues of diet-associated oral carcinogenesis--with an emphasis on areca-nut-induced oral cancer development[J]. J Oral Pathol Med, 2015, 44(3):167-177. doi: 10.1111/jop.12171 URL
[7]	Zhang J, Raju GS, Chang DW, et al. Global and targeted circulating microRNA profiling of colorectal adenoma and colorectal cancer[J]. Cancer, 2018, 124(4):785-796. doi: 10.1002/cncr.31062 URL
[8]	Kim MH, Cho JS, Kim Y, et al. Discriminating between terminal- and non-terminal respiratory unit-type lung adenocarcinoma based on microRNA profiles[J]. PLoS One, 2016, 11(8):e0160996.
[9]	Liang Z, Kong R, He Z, et al. High expression of miR-493-5p positively correlates with clinical prognosis of non small cell lung cancer by targeting oncogene ITGB1[J]. Oncotarget, 2017, 8(29):47389-47399. doi: 10.18632/oncotarget.17650 pmid: 28537888
[10]	Cinegaglia NC, Andrade SC, Tokar T, et al. Integrative transcriptome analysis identifies deregulated microRNA-transcription factor networks in lung adenocarcinoma[J]. Oncotarget, 2016, 7(20):28920-28934. doi: 10.18632/oncotarget.8713 pmid: 27081085
[11]	Feng H, Ge F, Du L, et al. MiR-34b-3p represses cell proliferation, cell cycle progression and cell apoptosis in non-small-cell lung cancer (NSCLC) by targeting CDK4[J]. J Cell Mol Med, 2019, 23(8):5282-5291. doi: 10.1111/jcmm.14404 URL
[12]	Swellam M, Zahran RFK, Ghonem SA, et al. Serum miRNA-27a as potential diagnostic nucleic marker for breast cancer[J]. Arch Physiol Biochem, 2019,1-7.
[13]	Wang Z, Wang J, Yang Y, et al. Loss of has-miR-337-3p expression is associated with lymph node metastasis of human gastric cancer[J]. J Exp Clin Cancer Res, 2013, 32(1):76. doi: 10.1186/1756-9966-32-76 URL
[14]	Kim SY, Lee YH, Bae YS. MiR-186, miR-216b, miR-337-3p, and miR-760 cooperatively induce cellular senescence by targeting α subunit of protein kinase CKⅡ in human colorectal cancer cells[J]. Biochem Biophys Res Commun, 2012, 429(3-4):173-179. doi: 10.1016/j.bbrc.2012.10.117 URL
[15]	Xiang X, Mei H, Zhao X, et al. miRNA-337-3p suppresses neuroblastoma progression by repressing the transcription of matrix metalloproteinase 14[J]. Oncotarget, 2015, 6(26):22452-22466. pmid: 26084291
[16]	Nagase H, Visse R, Murphy G. Structure and function of matrix metalloproteinases and TIMPs[J]. Cardiovasc Res, 2006, 69(3):562-573. doi: 10.1016/j.cardiores.2005.12.002 URL
[17]	Conlon GA, Murray GI. Recent advances in understan-ding the roles of matrix metalloproteinases in tumour inva-sion and metastasis[J]. J Pathol, 2019, 247(5):629-640. doi: 10.1002/path.5225
[18]	Dufour A, Sampson NS, Zucker S, et al. Role of the hemopexin domain of matrix metalloproteinases in cell migration[J]. J Cell Physiol, 2008, 217(3):643-651. doi: 10.1002/jcp.21535 URL
[19]	Remacle AG, Cieplak P, Nam DH, et al. Selective function-blocking monoclonal human antibody highlights the important role of membrane type-1 matrix metalloproteinase (MT1-MMP) in metastasis[J]. Oncotarget, 2017, 8(2):2781-2799. doi: 10.18632/oncotarget.13157 pmid: 27835863
[20]	Li Y, Kuscu C, Banach A, et al. miR-181a-5p inhibits cancer cell migration and angiogenesis via downregulation of matrix metalloproteinase-14[J]. Cancer Res, 2015, 75(13):2674-2685.
[21]	Momen-Heravi F, Bala S. Emerging role of non-coding RNA in oral cancer[J]. Cell Signal, 2018, 42:134-143. doi: S0898-6568(17)30277-2 pmid: 29056500
[22]	Huang SH, O'Sullivan B. Overview of the 8th edition TNM classification for head and neck cancer[J]. Curr Treat Options Oncol, 2017, 18(7):40. doi: 10.1007/s11864-017-0484-y URL
[23]	Zhuang Q, Shen J, Chen Z, et al. MiR-337-3p suppresses the proliferation and metastasis of clear cell renal cell carcinoma cells via modulating Capn4[J]. Cancer Biomark, 2018, 23(4):515-525. doi: 10.3233/CBM-181645 URL
[24]	Cao XM. Role of miR-337-3p and its target Rap1A in modulating proliferation, invasion, migration and apoptosis of cervical cancer cells[J]. Cancer Biomark, 2019, 24(3):257-267. doi: 10.3233/CBM-181225 URL
[25]	Yuan H, Wei R, Xiao Y, et al. RHBDF1 regulates APC-mediated stimulation of the epithelial-to-mesenchymal transition and proliferation of colorectal cancer cells in part via the Wnt/β-catenin signalling pathway[J]. Exp Cell Res, 2018, 368(1):24-36. doi: 10.1016/j.yexcr.2018.04.009 URL

特征	例数（n=20）	miRNA-337-3p		P值
特征	例数（n=20）	高表达(n=10)	低表达(n=10)	P值
性别				0.656
男	10	4	6
女	10	6	4
年龄（岁）				0.650
≤60	8	3	5
>60	12	7	5
吸烟^a）				0.582
是	4	1	3
否	16	9	7
饮酒^b）				0.211
是	3	0	3
否	17	10	7
T分期				0.350
T₁+T₂	13	5	8
T₃+T₄	7	5	2
N分期				0.029^*
N₀	7	6	1
N₁+N₂	13	4	9
病理分级				0.650
Ⅰ	8	5	3
Ⅱ+Ⅲ	12	5	7