Objective: To analyze the application value of noninvasive ocular surface analyzer in the diagnosis of dry eye. Methods: A total of 150 patients suspected of dry eye from December 2017 to December 2018 were enrolled. All the patients were examined with noninvasive ocular surface analyzer(non-invasive method), and traditional invasive examination was then performed. The detection rate of tear film abnormality was compared between the two methods. Pearson and Spearman analysis was used to analyze the relationship between results of noninvasive method and indice of dry eye. Results: The detection rate of tear film abnormality by non-invasive method (90.67%) was significantly higher than that by traditional invasive examination(74.67%)(P<0.05). Tear break-up time (TBUT) by traditional invasive examination was positively correlated with the noninvasive tear break-up time (NITBUT) (r=0.88, P<0.01）. Schirmer I test (SIT ) by traditional invasive examination was positively correlated with NITBUT（r=0.54, P<0.01）. Tear meniscus height (TMH) by non-invasivemethod was positively correlated with TBUT and SIT（r=0.53, P<0.01; r=0.76, P<0.01）. Scores of lipid layer ana-lysis and meibomian gland loss were negatively correlated with TBUT（r=-0.61, P<0.01）, but had no relationship with SIT（r=0.25, P=0.15）. Conclusions: Detection rate of tear film abnormality by non-invasive ocular surface analyzer is higher than that by traditional invasive examination, and can assist the classification and formulating of treatment of dry eye.