Journal of Diagnostics Concepts & Practice >

2020 , Vol. 19 >Issue 04: 414 - 419

DOI: https://doi.org/10.16150/j.1671-2870.2020.04.017

Original articles

Functional study and detection of vimentin produced by miR-200c target gene in human gastric cancer BGC823 cells

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  • 1. Department of Gastroenterology, Ruijin Hospital North, Shanghai Jiao Tong University School of Medicine, Shanghai 201801, China
    2. Department of Infection Control, the First Hospital of Jiujiang City, Jiangxi Jiujiang 332000, China
    3. Department of Laboratory, Maternity and Child Health Hospital of Jiujiang City, Jiangxi 332000, China
    4. Department of Pathogenic Biology, Bengbu Medical College, Anhui Bengbu 233030, China

Received date: 2019-07-09

  Online published: 2022-07-15

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Abstract

Objective: To investigate the expression of zinc finger E-box enhancer binding protein 2(ZEB2) gene in miR-200c in human gastric cancer BGC823 cells by RNA targeted interference technique, and detect expression of vimentin, so as to observe the effect of miR-200c on the function of gastric cancer BGC823 cells. Methods: The interference plasmids shZEB2 was constructed and transfected to BGC-823 cells in mediation of Lipofectamine 2000. The stable transfectants were screened by G418, and ZEB2 mRNA, miR-200c and vimentin was detected by real-time PCR in BCG823 cells, while protein expression levels of ZEB2 and vimentin by Western blotting. Proliferation of BGC-823 cells was evaluated by MTT assay. Transwell assay and wound healing assay were used to observe the invasion and migration ability of BCG823 cells, respectively. Results: With interfering ZEB2 expression, miR-200c expression level increased while proliferation, migration and invasion ability of BGC823 cells decreased significantly. Conclusions: ZEB2 may be the target of miR-200c, and the ability of BGC823 cells including proliferation, migration and invasion is significantly decreased after interference with ZEB2.

Key words： Gastric cancer; Zinc finger E-box enhancer binding protein 2 (ZEB2); Vimentin

Cite this article

YANG Cuiping, YANG Xiaojin, YANG Yanpin, ZHANG Mengyin, XIE Ling, YU Xiaojun, CAI Boer, CHEN Dengyu, CHEN Ping, WU Yunlin . Functional study and detection of vimentin produced by miR-200c target gene in human gastric cancer BGC823 cells[J]. Journal of Diagnostics Concepts & Practice, 2020 , 19(04) : 414 -419 . DOI: 10.16150/j.1671-2870.2020.04.017

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