Objective: To investigate the application of high performance liquid chromatography (HPLC) in the screening of thalassemia in Shanghai. Methods: The hemoglobin of 135 patients with suspected thalassemia were analyzed by using HPLC. Gene detection of thalassemia by GAP-PCR and reverse dot blot (RDB) were simultaneously performed. Results: Using gene detection as a gold standard, when HbA2>3.7% was taken as the cutoff value for diagnosis of β-thalassemia carriers by HPLC, the sensitivity and specificity were 100%, and 98.41%, and the positive predictive value and negative predictive value were 98.0% and 100%, respectively. If <1.7% was taken as the cutoff value of HbA2 for diagnosis of α-thalassemia (HbH disease) by HPLC, the indice above-mentioned were 100%, 100%, 100%, 100%, respectively. Out of 135 samples, there were 8 cases being detected a HbH peak. Conclusion: Results of HPLC detection are in excellent agreement with those of the gene detection in the diagnosis of β-thalassemia and HbH disease, while certain false negative rate may occur in the diagnosis of minor α-thalassemia or α-thalassemia trait.
WANG Yefei, WU Beiying, XIA Wenquan, HU Yiqun
. Application of high performance liquid chromatography (HPLC) in the screening of thalassemia in Shanghai[J]. Journal of Diagnostics Concepts & Practice, 2016
, 15(05)
: 472
-476
.
DOI: 10.16150/j.1671-2870.2016.05.008
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