Effect of high-dose glucocorticoid on glucose metabolism in rats

doi:10.16138/j.1673-6087.2021.06.006

Abstract

Abstract:

Objective To explore the effect of high-dose glucocorticoid(GC) on glucose metabolism in rats. Methods Choose appropriate dose in dexamethasone treatment through using different doses. By administering high-dose dexamethasone to Wistar rats, the effects of high-dose GC on rat body weight, fasting blood glucose, fasting insulin, glucose tolerance test, and insulin resistance test were observed. ¹⁸F-fluorodeoxyglucose(FDG) positron emission tomography (PET)/CT imaging was used to detect the effect of high-dose GC on glucose metabolism in skeletal muscle and liver of rats. Results Different doses of dexamethasone can cause changes in glucose metabolism, of which a dose of 10 mg/kg was suitable for this study. After high-dose dexamethasone treatment, the body weight of rats decreased[days 0,3,7,11,15 were (261±8)(226±8)(192±10)(172±10)(156±10) g, all P<0.000 1], fasting blood glucose [days 0,3,7,11,15 were(4.3±0.8)(14.4±5.2)(8.3±2.6)(9.8±4.4)(9.8±4.9) mmol/L, all P<0.05] and insulin levels increased[days 0,3,7,11,15 were (0.8±0.2)(11.6±1.1)(9.2±1.0)(9.2±2.4)(13.5±2.1) μg/L, all P<0.05], the area under the curve of glucose increased(days 0,3,7,11,15 were 858±26,2 350±345,1 680±331,1 352±166,1 553±217, all P<0.05), and insulin sensitivity decreased(days 0,3,7,11,15 were 1.26±0.18,0.51±0.09,0.91±0.18,0.77±0.16,0.50±0.16, all P<0.05). FDG uptake in skeletal muscle increased (days 0,3,7,11,15 were 0.10±0.01,0.15±0.03,0.20±0.02,0.28±0.02,0.27±0.03, all P<0.05), FDG uptake in liver didn’t change significantly, glycogen content in skeletal muscle and liver increased. Conclusions High-dose GC could cause significant hyperglycemia and hyperinsulinemia. Hyperinsulinemia compensated the insulin resistance of skeletal muscle caused by GC, however it could not completely compensate the deficiency of glucose metabolism in the liver.

Key words: Glucocorticoid, Glucose metabolism, Insulin resistance, Positron emission tomography/CT

CLC Number:

R587

ZHAO Qingqing, ZHOU Jinxin, PAN Yu, JU Huijun, ZHU Liying, LIU Yang, ZHANG Yifan. Effect of high-dose glucocorticoid on glucose metabolism in rats[J]. Journal of Internal Medicine Concepts & Practice, 2021, 16(06): 397-403.

Figures/Tables 5

References 32

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