Journal of Internal Medicine Concepts & Practice ›› 2023, Vol. 18 ›› Issue (06): 416-423.doi: 10.16138/j.1673-6087.2023.06.008
• Original article • Previous Articles Next Articles
HE Meijuan, HE Yanjie, WANG Yun, ZHU Chunxue, HUANG Hanpeng()
Received:
2023-02-06
Online:
2023-12-18
Published:
2024-03-18
CLC Number:
HE Meijuan, HE Yanjie, WANG Yun, ZHU Chunxue, HUANG Hanpeng. M2-sEV inhibit endoplasmic reticulum stress to alleviate chronic intermittent hypoxic-induced H9C2 cardiomyocyte injury[J]. Journal of Internal Medicine Concepts & Practice, 2023, 18(06): 416-423.
项目 | 正向引物 | 反向引物 |
---|---|---|
CD206 | 5’-GGTTCCGGTTTGTGGAGCAG-3’ | 5’-TCCGTTTGCATTGCCCAGTA-3’ |
Arg1 | 5’-TCCTTAGAGATTATCGGAGCG-3’ | 5’-GTCTTTGGCAGATATGCAGG-3’ |
TNF-α | 5’-ATGTCTCAGCCTCTTCTCATTC-3’ | 5’-GCTTGTCACTCGAATTTTGAGA-3’ |
IL-6 | 5’-CTCCCAACAGACCTGTCTATAC-3’ | 5’-CCATTGCACAACTCTTTTCTCA-3’ |
TGF-β | 5’-CCAGATCCTGTCCAAACTAAGG-3’ | 5’-CTCTTTAGCATAGTAGTCCGCT-3’ |
IRE1α | 5’-GCATCACCAAGTGGAAGTATC-3’ | 5’-ACCATTGAGGGAGAGGCATAG-3’ |
XBP1 | 5’-GGTCTGCTGAGTCCGCAGCACTC-3’ | 5’-AGGCTTGGTGTATACATGG-3’ |
活化的胱天蛋白酶3 | 5’-GCACTGGAATGTCAGCTCGCAA-3’ | 5’-GCCACCTTCCGGTTAACACGAG-3’ |
Bax | 5’-GGTTTCATCCAGGATCGAGCAGG-3’ | 5’-ACAAAGATGGTCACGGTCTGCC-3’ |
GAPDH | 5’-GCCAAGGCTGTGGGCAAGGT-3’ | 5’-TCTCCAGGCGGCACGTCAGA-3’ |
项目 | CON组 | CIH组 | CIH+M2-sEV组 | P |
---|---|---|---|---|
HIF-1α | 1 | 3.250±0.0391) | 2.250±0.0471)2) | <0.000 1 |
活化的胱天蛋白酶3 | 1 | 1.170±0.0331) | 1.030±0.0441)2) | <0.000 9 |
活化的胱天蛋白酶9 | 1 | 1.220±0.0231) | 1.090±0.0351)2) | <0.000 5 |
Bax/Bcl-2 | 0.450±0.019 | 2.210±0.0911) | 1.040±0.1061)2) | <0.000 1 |
IL-6 | 1 | 1.270±0.0331) | 1.080±0.0391)2) | <0.000 3 |
TNF-α | 1 | 1.470±0.0631) | 1.110±0.0771)2) | <0.000 7 |
TGF-β | 1 | 1.610±0.0481) | 0.097±0.0531)2) | <0.000 1 |
GRP78 | 1 | 1.530±0.0391) | 1.260±0.0501)2) | <0.000 1 |
ATF6 | 1 | 1.500±0.0531) | 1.170±0.0561)2) | <0.000 1 |
IRE1α | 1 | 2.370±0.0601) | 1.560±0.0611)2) | <0.000 1 |
XBP1 | 1 | 1.630±0.0631) | 1.210±0.0741)2) | <0.000 1 |
项目 | CON组 | CIH组 | CIH+M2-sEV组 | P |
---|---|---|---|---|
Bax | 1 | 2.190±0.0691) | 1.650±0.0801) 2) | <0.000 1 |
活化的胱天蛋白酶3 | 1 | 3.160±0.0421) | 1.740±0.0321) 2) | <0.000 1 |
IL-6 | 1 | 21.590±0.1961) | 9.270±0.3451) 2) | <0.000 1 |
TNF-α | 1 | 4.470±0.1051) | 2.190±0.1371) 2) | <0.000 1 |
TGF-β | 1 | 12.860±0.2031) | 5.590±0.2171) 2) | <0.000 1 |
IRE1α | 1 | 3.960±0.0521) | 2.150±0.1031) 2) | <0.000 1 |
XBP1 | 1 | 7.790±0.1521) | 3.040±0.1961) 2) | <0.000 1 |
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