Effect of external biliary drainage on lung inflammation and edema in severe acute pancreatitis rats

doi:10.16139/j.1007-9610.2018.01.014

Abstract

Abstract: Objective To investigate the roles of biliary tract external drainage(BTED) on lung inflammation and edema of the rats with severe acute pancreatitis(SAP). Methods Twenty-four SD rats were randomly divided into four groups of six each: group sham surgery(SS), group SAP, group SAP+BTED with the SAP rats established first and then BTED performed, group SAP+BTED+zinc protoporphyrin (ZnPP) with Znpp injected abdominally 24 h before and then SAP with BTED procedure. ZnPP is a specific inhibitor of heme oxygenase-1(HO-1), which can inhibit the expression of HO-1. The rats were sacrificed 24 h after surgery. The lung and blood were collected. The pathological changes of lung were scored by Schmidt criterion. mRNA expression of HO-1, TNT-α and IL-6 were detected by quantitative RT-PCR method, lung HO-1 expression by Western blot and myeloperoxidase(MPO) protein by ELISA. The ratio of dry/wet of lung tissue was calculated. Results The Schmidt score in group SAP+BTED were significantly lower than in group SAP(P<0.05). The expression of both TNT-α and IL-6 with MPO level in group SAP+BTED were significantly lower than in group SAP(P<0.05). But the dry/wet ratio and the expression of HO-1 protein in group SAP+BTED were significantly increased than in group SAP(P<0.05). All these effects induced by BTED were not present when ZnPP war given. Conclusions BTED could reduce the lung inflammation and edema of the rats with SAP via the HO-1 pathway.

Key words: External biliary drainage, Severe acute pancreatitis, Heme oxygenase-1

CLC Number:

R657.5⁺1

WANG Jinlong, LU Meiling, CHEN Ying, ZHAO Bing, MAO Enqiang, CHEN Erzhen. Effect of external biliary drainage on lung inflammation and edema in severe acute pancreatitis rats[J]. Journal of Surgery Concepts & Practice, 2018, 23(01): 62-66.

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