Analysis of effect of storage time and centrifugation speed on activity of biliary α-amylase

doi:10.16139/j.1007-9610.2020.06.006

Abstract

Abstract:

Objective To study the effect of storage time and centrifugation speed on the activity of biliary α-amylase and the factors which affect the activity of α-amylase. Methods A total of 18 cases were randomly selected from 27 cases with endoscopic retrograde cholangiopancreatography in our hospital. The activity of biliary α-amylase was measured by iodine-starch colorimetry after bile samples were stored at 4℃ for 30 min, 3 h, 12 h, 24 h, 72 h and 30 d respectively, which was to study storage time in 6 groups each 18 cases. The bile samples in other 9 cases were used to study centrifugation speed including one group without sample centrifugation and the other two groups with sample centrifugation at 3 000 r/min or 8 000 r/min respectively. Results The activity of α- amylase decreased 0.73%, 2.11% and 3.94% in the bile stored at 4℃ for 3 h, 12~72 h or stored to 30 d respectively when compared with that stored 30 min (P>0.05), which indicated that the activity of biliary α-amylase did not change significantly for a period of storage 30 d at 4℃. The activity of biliary α- amylase decreased 0.16% after the centrifugation of 3 000 r/min compared with that without centrifugation, and increased 4.74% after the centrifugation of 8 000 r/min. The increase of 4.91% in the activity of biliary α-amylase was found at 8 000 r/min compared with that at 3 000 r/min(P>0.05), which indicated that the activity of biliary α-AMY had no obvious change when the centrifugal speed did not exceed 8 000 r/min. Conclusions The activity of biliary α-amylase would be relatively stable when bile is stored at 4℃ for 30 d and the centrifugal speed is 8 000 r/min or less.

Key words: Biliary α-amylase, Storage time, Centrifugation speed, Stability, Iodine-starch colorimetry

CLC Number:

R657.42

LI Guangming, LIU Jiayue, ZHANG Guixin, LI Shijie, SHANG Dong, CHEN Hailong, YANG Yulong. Analysis of effect of storage time and centrifugation speed on activity of biliary α-amylase[J]. Journal of Surgery Concepts & Practice, 2020, 25(06): 473-476.

Figures/Tables 3

References 24

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时间	胆汁α淀粉酶活力（U/L）	与30 min组比较变化幅度	P值
30 min	426.7±128.4	/	/
3 h	423.6±122.9	-0.73	0.71
12 h	416.6±121.8	-2.37	0.37
24 h	418.3±136.3	-1.97	0.47
72 h	418.2±135.2	-2.00	0.42
30 d	409.9±129.7	-3.94	0.33

分组	胆汁α淀粉酶活力(U/L)	与不离心组比较变化幅度	与离心3 000 r/min组比较变化幅度
不离心	489.63±166.54	/	+2.70个百分点
离心3 000 r/min	476.42±148.81	-2.70个百分点	/
离心8 000 r/min	445.67±226.51	-8.98个百分点	-6.45个百分点

分组	胆汁α淀粉酶活力(U/L)	与不离心组比较变化幅度	与离心3 000 rpm组比较变化幅度
不离心	474.50±173.84	/	+0.16个百分点
离心3 000 r/min	473.73±160.52	-0.16个百分点	/
离心8 000 r/min	496.98±187.84	+4.74个百分点	+4.91个百分点