Journal of Surgery Concepts & Practice >

2021 , Vol. 26 >Issue 03: 262 - 268

DOI: https://doi.org/10.16139/j.1007-9610.2021.03.018

Original article

Proteomics analysis of exosome in mice with Candida albicans infection for biomarker

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  • Department of Trauma-Emergency & Critical Care Medicine, Shanghai Fifth People's Hospital, Fudan University, Shanghai 200240, China

Received date: 2021-02-20

  Online published: 2022-08-03

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Abstract

Objective To analyze serum exosome using proteomic in mice with Candida albicans infection and to find out marker. Methods Mice model of Candida albicans infection was established by injection of Candida albicans liquid through tail vein. Exosome in serum were extracted and analyzed by liquid chromatography-mass spectrometry/mass spectrometry. Mice models of both Staphylococcus aureus infection and Escherichia coli infection were established to compare the protein expression in exosome among three groups of infection. Results A total of 4 055 peptides and 418 proteins were identified. With the ratio <0.5 or >2.0 times between Candida albicans infection group and normal control group and with P<0.05, 10 of different proteins were screened out. Both Cathepsin B and Pentraxin 3 which were related with Candida albicans infection were identified by ELISA. Receiver operating characteristic curves were used to evaluate proteins and the different proteins in exosome were found to have high sensitivity and specificity. In 3 groups of Candida albicans infection, Staphylococcus aureus infection and Escherichia coli infection, it was found that exosome proteins expressed more in Candida albicans infection group and less in the other two infection group. Conclusions Proteins including Cathepsin B and Pentraxin 3 were related to Candida albicans infection, which could be used as molecular markers of Candida albicans infection.

Key words： Proteomics; Biomarker; Exosome; Candida albicans infection

Cite this article

WANG Zetian, WU Chunrong, QI Yue, XU Dan, SUN Keyuan, TANG Jianguo . Proteomics analysis of exosome in mice with Candida albicans infection for biomarker[J]. Journal of Surgery Concepts & Practice, 2021 , 26(03) : 262 -268 . DOI: 10.16139/j.1007-9610.2021.03.018

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