Proteomics analysis of exosome in mice with Candida albicans infection for biomarker

doi:10.16139/j.1007-9610.2021.03.018

Abstract

Abstract:

Objective To analyze serum exosome using proteomic in mice with Candida albicans infection and to find out marker. Methods Mice model of Candida albicans infection was established by injection of Candida albicans liquid through tail vein. Exosome in serum were extracted and analyzed by liquid chromatography-mass spectrometry/mass spectrometry. Mice models of both Staphylococcus aureus infection and Escherichia coli infection were established to compare the protein expression in exosome among three groups of infection. Results A total of 4 055 peptides and 418 proteins were identified. With the ratio <0.5 or >2.0 times between Candida albicans infection group and normal control group and with P<0.05, 10 of different proteins were screened out. Both Cathepsin B and Pentraxin 3 which were related with Candida albicans infection were identified by ELISA. Receiver operating characteristic curves were used to evaluate proteins and the different proteins in exosome were found to have high sensitivity and specificity. In 3 groups of Candida albicans infection, Staphylococcus aureus infection and Escherichia coli infection, it was found that exosome proteins expressed more in Candida albicans infection group and less in the other two infection group. Conclusions Proteins including Cathepsin B and Pentraxin 3 were related to Candida albicans infection, which could be used as molecular markers of Candida albicans infection.

Key words: Proteomics, Biomarker, Exosome, Candida albicans infection

CLC Number:

R519.3

WANG Zetian, WU Chunrong, QI Yue, XU Dan, SUN Keyuan, TANG Jianguo. Proteomics analysis of exosome in mice with Candida albicans infection for biomarker[J]. Journal of Surgery Concepts & Practice, 2021, 26(03): 262-268.

Figures/Tables 4

References 25

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蛋白质 ID	蛋白质名称	基因名称	肽段数	比值（白念珠菌组/正常对照组）	P值
D3ZT94	Pentraxin 3	Ptx3	9	8.546177	2.25E-05
H6X2X0	reactive protein	Crp	7	2.333257	0.000325
A0A0H2UHI5	Serine protease inhibitor A3N	Serpina3n	28	4.053251	2.71E-05
P20059	Hemopexin	Hpx	27	2.442018	0.000254
Q5BKC4	Complement component C9	C9	28	2.748338	4.88E-05
Q6IN22	Cathepsin B	Ctsb	3	3.425430	0.034993
P27139	Carbonic anhydrase 2	Ca2	6	8.901092	4.17E-06
P06238	Alpha-2-macroglobulin	A2m	82	11.166290	5.72E-06
A0A0H2UHF8	Alpha-1-acid glycoprotein	Orm1	7	16.030460	5.07E-10
Q7TQ70	Fibrinogen alpha chain	Fga	26	3.043354	4.64E-05