木霉菌REMI突变菌株在降解敌敌畏中的作用

doi:10.3969/J.ISSN.1671-9964.2016.01.015

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Abstract

The dichlorvos degradation abilities of Trichoderma koningii mutants obtained by REMI (restriction enzyme mediated integration) and the optimal conditions for effective degradation were studied.Results showed both the wild type (T30) and mutants (TK-1, TK-2, TK-3, TK-5, TK-7, TK-8, TK-21, TK-38, TK-53, TK-30, TK-42)were able to degrade dichlorvos.TK-3 could reach 98% dichlorvos decomposition, which is the most efficient among mutants.Dichlorvos degradation rate was closely associated with glucose, dichlorvos initial concentration and pH of the medium.It was found that glucose at 1 000 μg/mL, pH 7.0 and dichlorvos at 500 μg/mL were the optimal conditions for effectively degrading dichlorvos by the mutant TK-3.The degradation efficiency of TK-3 would decline if dichlorvos concentration exceeded 1 000 μg/mL.

Key words： Trichoderma koningii restriction enzyme mediated integration (REMI) dichlorvos degradation

Received: 13 November 2014 Published: 08 March 2016

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	Articles by authors
	Xu YUAN
	Li-ping JIAN
	Jie CHEN

Cite this article:

Xu YUAN,Li-ping JIAN,Jie CHEN. Dichlorvos Degradation by REMI Mutants of Trichoderma koningii[J]. Journal of Shanghai Jiaotong University (Agricultural Sciences), 2016, 34(1): 85-90.

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http://www.qk.sjtu.edu.cn/jsjtua/EN/10.3969/J.ISSN.1671-9964.2016.01.015 OR http://www.qk.sjtu.edu.cn/jsjtua/EN/Y2016/V34/I1/85

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