组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2008, Vol. 4 ›› Issue (6): 312-314.doi: 10.3969/j.issn.1673-0364.2008.06.004

• 论著 • 上一篇    下一篇

体外扩增后人脂肪源干细胞的体外免疫调节功能的实验研究

白雯，江明，崔磊，尹烁   

  1. 新疆医科大学第一附属医院血液科;上海组织工程研究与开发中心;
  • 发布日期:2020-07-23

An Experimental Study for the Immunological Properties of Human Adipose-derived Stem Cells After Expansion

BAI Wen1,JIANG Ming1,CUI Lei2,YIN Shuo2   

  1. 1 Department of Blood;First Hospital Affiliated Xinjiang Medical University;Wulumuqi 830054;China;2 Shanghai Tissue Engineering Research and Development Center;Shanghai 200235;China.;
  • Published:2020-07-23

摘要： 目的研究体外扩增后人脂肪源干细胞(Adipose-derived stem cells,ADSCs)在体外的免疫调节功能,以期以少量组织获得足够量的低免疫原性且具有免疫调节功能的脂肪源干细胞,用于组织修复及细胞治疗。方法流式细胞仪检测扩增至第2代及第5代的人脂肪源干细胞表面标记物,观察在扩增的过程中ADSCs表面标记物的变化;同时,检测扩增至第5代的ADSCs在体外抑制淋巴细胞增殖的能力,以评价ADSCs的免疫原性及免疫调节功能。结果ADSCs在扩增过程造血干细胞标记物表达降低,间质细胞表型及内皮细胞表型变化无显著性差异,同时第5代的ADSCs在体外不会引起淋巴细胞的增殖,并且在混合淋巴细胞反应中可以抑制淋巴细胞的增殖。结论扩增中的ADSCs逐渐表达均一的间充质干细胞的表面标记物,并在扩增至第5代时仍保持有低免疫原性及免疫调节的能力。

关键词: 脂肪源干细胞, 混合淋巴细胞反应, 扩增

Abstract: Objective To study the immunological properties of human adipose-derived stem cells (ADSCs) with passage in vitro, in order to have enough stem cells with low immunogenicity and immune regulation function for the use in the organization repair and cell therapy. Methods Flow cytometry was performed to show the changes with the passage of ADSCs, and its ability to inhibit proliferation of lymphocyte with the fifth passage of ADSCs in vitro. Results The expression of the stromal-associated markers and endothel...

Key words: Adipose-derived stem cells, Mixed-Lymphocyte culture test, Expansion

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