组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2009, Vol. 5 ›› Issue (3): 143-147.doi: 10.3969/j.issn.1673-0364.2009.06.007

• 论著 • 上一篇    下一篇

聚左旋丙交酯纳米纤维支架与大鼠脊髓组织相容性的研究

张燕青，曾园山，何留民，全大萍，李燕   

  1. 中山大学中山医学院组织胚胎学教研室神经科学研究室;中山大学化学与化学工程学院高分子研究所;
  • 发布日期:2020-07-23

Research Biocompatibility of the Poly(l-lactic acid) Nano-fibrous Scaffolds with Spinal Cord Tissues in Rats

ZHANG Yanqing1,ZENG Yuanshan1,HE Liumin2,QUAN Daping2,LI Yan1   

  1. 1 Division of Neuroscience; Department of Histology and Embryology;Zhongshan School of Medicine;Sun Yat-sen University;Guangzhou 510080;China;2 Institute of Polymer Science;School of Chemistry and Chemical Engineering;Guangzhou 510275;China.;
  • Published:2020-07-23
  • Contact: 国家自然科学基金(30771143);;广东省自然科学基金(07117373)

摘要： 目的探讨聚左旋丙交酯[Poly(l-lactic acid),PLLA]纳米纤维支架与大鼠脊髓组织的相容性。方法以PLLA为原料,采用液-液相分离技术构建纳米纤维支架(Nanofibrous scaffolds)。在体外构建骨髓间充质干细胞(MSCs)-PLLA纳米纤维支架复合体;将12只SD大鼠随机分为两组,即损伤对照组和MSCs移植组。暴露大鼠胸10脊髓段做半横断损伤,其中在损伤对照组脊髓损伤处移植PLLA纳米纤维支架,在MSCs移植组脊髓损伤处移植MSCs-PLLA纳米纤维支架复合体。术后两组动物观察60d。HE染色观察结构改变,免疫组织化学方法观察炎症细胞及MSCs的存活情况。结果体外成功构建MSCs-PLLA纳米纤维支架复合体。扫描电镜显示,PLLA纳米纤维支架由相互贯穿的微孔组成。荧光显微镜和扫描电镜下均可见MSCs能够很好地黏附在支架上,分布较均匀。移植术后60d,HE染色显示,PLLA纳米纤维支架与脊髓组织融合较好,无明显空洞和瘢痕。CD68免疫组织化学显示,在移植物与宿主脊髓交界处有少量阳性细胞,表明有轻微炎症反应。GFP免疫组织化学显示,移植在脊髓损伤处的PLLA纳米纤维支架内有大量阳性M...

关键词: 聚左旋丙交酯, 纳米纤维支架, 骨髓间充质干细胞, 脊髓损伤, 生物相容性

Abstract: Objective To explore the biocompatibility of the Poly(l-lactic acid)(PLLA) nano-fibrous scaffold with spinal cord tissue of rats.Methods PLLA three-dimensional(3D) scaffolds with macro/micro-pores and nano-fibrous structure were fabricated by phase separation from a ternary PLLA/dioxane/water system.MSCs-PLLA nano-fibrous scaffolds complex were constructed in vitro.Twelve SD rats were divided into two groups:control injured group and MSCs transplanted group.T10 spinal cord segment was exposed,and then trans...

Key words: PLLA, Nano-fibrous scaffold, Bone marrow mesenchymal stem cells, Spinal cord injury, Biocompatibility

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