组织工程与重建外科杂志 ›› 2019, Vol. 15 ›› Issue (5): 318-334.doi: 10.3969/j.issn.1673-0364.2019.05.002

• 论著 • 上一篇    下一篇

猪骨髓间充质干细胞体外构建软骨修复关节软骨缺损的实验研究

宋楠,何爱娟   

  1. 复旦大学附属眼耳鼻喉科医院眼耳鼻整形外科
  • 收稿日期:2019-06-17 修回日期:2019-08-23 出版日期:2019-10-20 发布日期:2019-10-20
  • 作者简介:何爱娟(E-mail:heaijuan2013@163.com)。

Repair of Articular Cartilage Defects Using Tissue Engineered Cartilage Constructed by Pig’s BMSCs in Vitro

SONG Nan,HE Aijuan   

  1. Department of Facial Plastic and Reconstructive Surgery, Eye And ENT Hospital, Fudan University
  • Received:2019-06-17 Revised:2019-08-23 Online:2019-10-20 Published:2019-10-20

摘要: 目的探讨骨髓间充质干细胞(Bone marrow mesenchymal stem cells, BMSC)体外构建软骨,移植修复大动物大面积关节软骨缺损的可行性。方法以7只长枫杂交猪为动物模型,建立软骨缺损模型,用自体BMSCs体外软骨定向诱导8周的组织工程软骨移植修复,以单纯材料组、空白对照组和正常对照组作为对照。术后6个月取材,行大体及组织学检测,同时于术前、术后抽取关节液,检测IGF-1、IL-1β、MMP-13及TNF-α的表达水平,综合评价修复效果。结果术后6个月取材,实验组获得了较理想的修复效果,修复区软骨组织大体观、组织学表现成熟,类似于正常软骨组织。关节液检测中,实验组IGF-1表达水平显著高于空白对照组及单纯材料组(P<0.05),而IL-1β、MMP-13及TNF-α的表达水平则明显低于空白对照组及单纯材料组(P<0.05)。结论 BMSC体外构建组织工程软骨可有效修复猪关节软骨缺损,从而避免或延缓骨关节炎的发生、发展。

关键词: 组织工程, 骨髓间充质干细胞, 关节软骨缺损

Abstract: Objective To investigate the feasibility of bone marrow mesenchymal stem cells(BMSCs) in vitro cartilage construction and transplantation for repairing large articular cartilage defects in large animals. Methods The cartilage defect model was established by using 7 changfeng hybrid pigs as animal models, and the cartilage defect was repaired by tissue engineered cartilage transplantation with autologous BMSCs in vitro targeted induction for 8 weeks. The pure PGA/PLA material group, the blank control group and the normal cartilage group were set up as control. Six months after operation,gross and histological examinations were carried out. At the same time, the expression levels of the key cytokines such as IGF-1, IL-1β, MMP-13 and TNF-α in synovial fluid were detected before and after operation. The repair effect was evaluated comprehensively. Results Six months after the operation, most of the experimental groups obtained ideal cartilage tissue repair, the general view and histological maturity of the cartilage tissue in the repair area was very close to the normal cartilage tissue. In the test of joint fluid, the expression of IGF-1 in the experimental group was significantly higher than that in the blank control group and PGA group(P <0.05), while the expression levels of IL-1β, MMP-13 and TNF-α were significantly lower than those in the blank control group and PGA group(P<0.05). Conclusion Tissue engineered cartilage constructed by pig’s BMSCs in vitro can effectively repair articular cartilage defects, thus avoiding or delaying the development of osteoarthritis.

Key words: Tissue engineering, Bone marrow mesenchymal stem cells, Articular cartilage defects

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