组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2017, Vol. 13 ›› Issue (5): 287-298.doi: 10.3969/j.issn.1673-0364.2017.05.013

• 论著 • 上一篇    下一篇

趋化因子SDF-1促进基于内源性干细胞归巢的初步研究

黄晓，陈晖，刘玉健，汪俊   

  1. 上海市口腔病防治院儿童口腔科；上海市口腔医学重点实验室，上海市 上海交通大学医学院附属第九人民医院儿童口腔科
  • 收稿日期:2017-07-16 发布日期:2020-07-23

A Preliminary Study of SDF-1 in Stem Cell Homing-guided Tissue Regeneration

HUANG Xiao,CHEN Hui,LIU Yujian,WANG Jun   

  • Received:2017-07-16 Published:2020-07-23

摘要： 目的 通过体内、外实验,研究基质细胞衍生因子1(Stromal cell–derived factor-1,SDF1)对细胞的趋化作用。方法 体外培养SD大鼠骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs)。通过Transwell实验,检测不同浓度SDF1对BMSC体外趋化作用的影响。采用MTT法,检测不同浓度SDF1对BMSC细胞活性的影响。通过将负载SDF1的无纺聚羟基乙酸植入裸鼠皮下,检测SDF1的体内趋化活性。结果10 ng/m L、50 ng/m L、100 ng/m L、200 ng/m L SDF1组体外趋化BMSC数目均较对照组增加,其中100 ng/m L SDF1组趋化细胞数目最高。10 ng/m L、50 ng/m L、100 ng/m L SDF1对BMSC细胞活性无影响,200 ng/m L SDF1可降低BMSC细胞活性。100 ng/m L SDF1可促进体内细胞募集。结论100 ng/m L SDF1可有效促进细胞趋化且对细胞活性无影响。

关键词: 基质细胞衍生因子, 1, 骨髓间充质干细胞, 干细胞募集, 趋化作用

Abstract: Objective To investigate the chemotaxis of stromal cell derived factor-1 (SDF1) in vitro and in vivo. Methods Bone marrow mesenchymal stem cells (BMSCs) were cultivated from SD rats. Transwell test was used to evaluate the influences of different concentration of SDF1 on chemotaxis of BMSC. MTT test was used to analyze the effects of different concentration of SDF1 on the cell viability of BMSC. 100 ng/mL SDF1-loaded PLGA was implanted in nude mice to study the the chemotaxis of SDF1 in vivo. Results All 4 groups of SDF1 (10 ng/mL, 50 ng/mL, 100 ng/mL and 200 ng/mL) recruited more BMSC than the control group, and 100 ng/mL SDF1 showed better results than the other groups. 200 ng/mL SDF1 deceased cell viability of BMSC on day 1, 4 and 7, while 10 ng/mL, 50 ng/mL and 100 ng/mL SDF1 had no effects on the the viability of BMSC. In vivo analysis showed that 100 ng/mL SDF1 could promote cell recruitment. Conclusion 100 ng/mL SDF1 could effectively promote BMSC recruitment without influence on cell viability in vitro and 100 ng/mL SDF1 could promote cell homing in vivo.

Key words: Stromal cell derived factor-1, Bone marrow mesenchymal stem cells, Stem cell recruitment, Chemotaxis

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