组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2016, Vol. 12 ›› Issue (6): 331-334.doi: 10.3969/j.issn.1673-0364.2016.06.001

• 论著 • 上一篇    下一篇

金纳米团簇标记PLGA支架的研究

王祥盛，王贤松，张智勇，周广东，刘伟，曹谊林，张文杰   

  1. 上海交通大学医学院附属第九人民医院整复外科上海市组织工程研究重点实验室；组织工程国家工程中心
  • 发布日期:2020-07-23

Labeling PLGA Scaffold with Gold Nanoclusters

WANG Xiangsheng,WANG Xiansong,ZHANG Zhiyong,ZHOU Guangdong,LIU Wei,CAO Yilin,ZHANG Wenjie   

  1. Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering;National Tissue Engineering Center of China;
  • Published:2020-07-23
  • Contact: 国家自然科学基金（81271714,31170944）；上海市科委基础重点研究项目（15jc1490600）

摘要： 目的探讨金纳米团簇用于示踪可降解高分子支架的可行性,为非侵入成像方式监测体内组织工程支架降解提供方法和思路。方法用氯金酸和牛血清白蛋白(BSA)为原料合成金纳米团簇(Au Nanoclusters,Au NCs),进行相关表征及细胞毒性检测。以Au NCs标记聚乳酸-羟基乙酸共聚物(PLGA),制备Au NCs/PLGA支架,扫描电镜检测支架表面结构,荧光及CT扫描检测支架特性。将支架植入裸鼠皮下,应用荧光成像及Micro-CT扫描,以观测支架在体内的情况。结果合成的Au NCs具有荧光特性,无细胞毒性;纳米团簇标记的PLGA支架保持荧光特性,同时在CT扫描中可显影;Au NCs/PLGA支架可以在裸鼠皮下通过荧光成像及Micro-CT扫描来示踪。结论 Au NCs可作为组织工程支架的示踪剂,为非侵入成像方式监测体内高分子组织工程支架降解情况提供可能。

关键词: 金纳米团簇, 支架材料, 非侵入成像

Abstract: Objective To explore the feasibility of using Au nanoclusters （AuNCs） to label the tissue engineering scaffold which degradation could be further monitored by noninvasive approach in vivo. Methods Chlorauric acid and bovine serum albumin （BSA） were used to synthesize AuNCs. The nanoclusters were characterized and their cytotoxicity was evaluated. AuNCs were then mixed with poly lactic-co-glycolic acid （PLGA） to fabricate AuNCs/PLGA scaffold. The scaffolds were implanted subcutaneously in nude mice. Fluorescence imaging and CT scanning were performed to detect the scaffolds in vivo. Results Synthesized AuNCs possess fluorescence without cytotoxicity. The AuNCs labeled PLGA scaffolds were detected by fluorescence imaging and CT scanning in vitro. The scaffolds were detected by these two noninvasive methods after being implanted in nude mice. Conclusion AuNCs could be potentially used for labeling polymer scaffold, which degradation could be possibly monitored by non-invasive imaging technology.

Key words: Au nanoclusters, Scaffold, Non-invasive imaging

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