组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2019, Vol. 15 ›› Issue (4): 222-244.doi: 10.3969/j.issn.1673-0364.2019.04.002

• 论著 • 上一篇    下一篇

软骨脱细胞基质仿生支架体外构建组织工程软骨

贾立涛，姚琳，刘延群，张沛灵，刘豫，曹谊林，周广东   

  1. 潍坊医学院整形外科研究所；组织工程国家工程研究中心；上海交通大学医学院附属第九人民医院整复外科，上海市组织工程研究重点实验室
  • 收稿日期:2019-06-03 修回日期:2019-07-19 出版日期:2019-08-20 发布日期:2019-08-20
  • 作者简介:周广东(E-mail:guangdongzhou@126.com)。

In Vitro Reconstruction of Tissue Engineering Cartilage Using Acellular Cartilage Matrix Biomimetic Scaffolds

JIA Litao,YAO Lin,LIU Yanqun,ZHANG Peiling,LIU Yu,CAO Yilin,ZHOU Guangdong   

  • Received:2019-06-03 Revised:2019-07-19 Online:2019-08-20 Published:2019-08-20
  • Contact: 国家重点研发计划(2017YFC1103900)；国家自然科学基金项目(81871502，81701843)

摘要： 目的探索软骨脱细胞基质(ACM)仿生支架体外构建组织工程软骨的可行性。方法将软骨组织彻底粉碎后脱细胞处理,复合一定比例的明胶(GT)制备三维多孔支架。分析比较不同ACM/GT比例多孔支架的孔径、孔隙率、生物力学和降解速率,选取最适宜软骨体外再生的一组多孔支架用于体外构建。获取、培养猪关节软骨细胞,接种于ACM多孔支架上,体外培养8周后,行大体观察及组织学检测。结果 ACM多孔支架孔隙分布均匀,随ACM含量的增加,孔径和孔隙率逐渐增大,力学强度逐渐降低,其中,ACM:GT=5:5组在孔径和孔隙率方面均适宜体外软骨再生。大体观察和组织学检测显示,ACM支架可体外再生均质、典型的软骨组织。结论 ACM可制成适宜软骨再生的三维多孔支架,并可体外构建组织工程软骨。

关键词: 软骨脱细胞基质, 明胶, 支架材料, 软骨再生, 组织工程软骨

Abstract: Objective To explore the feasibility of constructing tissue engineering cartilage with acellular cartilage matrix (ACM) biomimetic scaffolds in vitro. Methods The cartilage tissue was decellularized after completely pulverization, and then combined with a certain proportion of gelatin (GT) to prepare three-dimensional porous scaffolds. The pore size, porosity, biomechanics and degradation rate of porous scaffolds with different ACM/GT ratios were analyzed, and the most suitable proportion for cartilage regeneration in vitro was selected. Porcine articular cartilage cells were cultured, and then inoculated into ACM scaffolds. Gross observation and histological staining were carried out after culturing for 8 weeks in vitro. Results With the increase of ACM content, the pore size and porosity of ACM scaffolds increased while the biomechanical strength decreased. In terms of pore size and porosity, the ACM:GT=5:5 one was suitable for cartilage reconstruction. Gross observation and histology showed that ACM scaffolds could reconstruct homogeneous and typical cartilage tissue in vitro. Conclusion ACM can be used to fabricate three-dimensional porous scaffolds that are suitable for cartilage regeneration and to construct tissue engineering cartilage in vitro.

Key words: Acellular cartilage matrix, Gelatin, Scaffold, Cartilage regeneration, Tissue engineering cartilage

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