组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2019, Vol. 15 ›› Issue (1): 13-16.doi: 10.3969/j.issn.1673-0364.2019.01.004

• 论著 • 上一篇    下一篇

SVF-gel促进糖尿病鼠创面愈合的初步实验研究

蒯权，王宜梅，李聪，黄娜，祝愿，文辉才   

  1. 南昌大学第一附属医院整形科
  • 收稿日期:2018-11-03 修回日期:2018-12-26 出版日期:2019-02-20 发布日期:2019-02-20
  • 作者简介:文辉才(E-mail:whcjxmc@163.com)。

Preliminary Study of SVF-gel Promoting Wound Healing in Diabetic Rats

KUAI Quan,WANG Yimei,LI Cong,HUANG Na,ZHU Yuan,WEN Huicai   

  1. Department of Plastic Surgery,The First Affiliated Hospital of Nanchang University,Nanchang 330006,China)
  • Received:2018-11-03 Revised:2018-12-26 Online:2019-02-20 Published:2019-02-20

摘要： 目的 探讨SVF-gel治疗糖尿病大鼠慢性创面的效果及机制。方法 36只雄性SD大鼠,采用一次性腹腔注射链脲佐菌素(STZ)55 mg/Kg制备糖尿病模型,1周后于糖尿病鼠背部两侧各制备1个直径为1.2 cm的圆形全层皮肤缺损创面。随机分为3组,每组12只,分别采用SVF-gel(A组)、SVF悬液(B组)、生理盐水(C组)处理创面。观察各组创面愈合情况并计算创面愈合率,采用HE染色、免疫组织化学染色观察创面内肉芽组织形态学改变和新生血管情况。结果 A、B组各时间点创面愈合率均优于C组(P<0.05),A组各时间点创面愈合率均优于B组(P<0.05)。相比C组,A、B组炎症细胞浸润较少,再上皮化程度明显,新生血管明显增多,肉芽组织结构致密,且A组组织学改变最为明显。结论 SVF-gel治疗可以通过减轻炎症反应,促进创面内血管生成,增加肉芽组织形成来加速慢性创面的愈合。

关键词: 血管基质组分, 慢性创面, 创面修复

Abstract: Objective To investigate the effect and mechanism of SVF-gel promoting wounds healing in diabetic rats.Methods Thirty-six male SD rats were prepared by intraperitoneal injection of Streptozotoein(55 mg/Kg)to establish the diabetic model.The wound with a diameter of 0.6 cm was made on both sides of diabetic rats after one week.Then those rats were divided into 3 groups(n=12)randomly,and treated with SVF-gel(Group A),SVF(Group B),and normal saline(Group C)respectively.The wound sites were observed and the percentage of wound closure was calculated.HE staining and immunohistochemical staining were used to observe the morphological changes of granulation tissue in the wound and the angiogenesis.Results The wound healing rates at all time points of group A and group B were higher than that of group C(P<0.05),and the wound healing rates at all time points of group A were higher than that of group B(P<0.05).Histological changes:Compared with group C,the infiltration of inflammatory cells was decreased in group A and group B,the degree of reepithelialization was obvious,the angiogenesis was significantly increased,the granulation tissue structure was dense,and these histological changes were the most obvious in group A.Conclusion For the wounds of diabetic mice,SVF-gel may increase the formation of blood vessels and the granulation tissue,reduce the inflammatory reaction to promote the wound healing.

Key words: Stromal vascular fraction, Chronic wound, Wound repair

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