组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2019, Vol. 15 ›› Issue (4): 227-232.doi: 10.3969/j.issn.1673-0364.2019.04.003

• 论著 • 上一篇    下一篇

Mmu-miR-124靶基因预测及通过GSK3B调控神经发育的可能性：基于生物信息学和qPT-PCR分析

蒋云凤，孙宇   

  1. 上海交通大学医学院附属第九人民医院麻醉科
  • 收稿日期:2019-05-15 修回日期:2019-07-03 出版日期:2019-08-20 发布日期:2019-08-20
  • 作者简介:孙宇(E-mail:dr_sunyu@163.com)。

Mmu-miR-124 Target Gene Prediction and Its Potential Regulation of Neurodevelopment Through GSK3B: A Bioinformatics and qRT-PCR Study

JIANG Yunfeng,SUN Yu   

  • Received:2019-05-15 Revised:2019-07-03 Online:2019-08-20 Published:2019-08-20
  • Contact: 上海市科学技术委员会资助项目(18ZR1422900)

摘要： 目的运用生物信息学手段预测Mmu-miR-124靶基因,并进一步揭示Mmu-miR-124在神经发育中的作用。方法从StarBase数据库中预测出Mmu-miR-124的潜在靶基因。运用GO、Reactome通路、KEGG通路和PPI分析方法,对预测的靶基因的可能机制进行分析。采用逆转录实时荧光定量PCR(qRT-PCR)技术验证神经发育通路关键基因预测结果的可信度。结果 GO、KEGG与Reactome通路提示,Mmu-miR-124靶基因在轴突导向、长时程压抑和鞘脂类代谢通路中发挥作用;PPI分析表明,GSK3B、NRAS和ITGB1是关键基因;qRT-PCR结果显示,在神经发育过程中Mmu-miR-124表达明显升高,GSK3B表达略下降。结论 Mmu-miR-124通过调控靶基因广泛参与多种生物学过程,Mmu-miR-124可能通过靶向GSK3B调控神经发育。

关键词: Mmu-miR-124, 靶基因, 生物信息学分析, 神经发育

Abstract: Objective To predict the target genes of Mmu-miR-124 by bioinformatics method, and to identify the role of Mmu-miR-124 in neurodevelopment. Methods The potential target genes of Mmu-miR-124 were predicted on Star Base database. GO, Reactome Pathway, KEGG Pathway and PPI analysis methods were used to analyze the possible mechanism of the predicted target genes. The reliability of the predicted results on key genes in neural developmental pathways was confirmed by real-time fluorescence quantitative reverse transcriptional PCR (qRT-PCR) technique. Results GO, KEGG and Reactome pathway suggested the role of Mmu-miR-124 target genes in axon guidance, long-term depression and sphlein metabolic pathway. PPI analysis showed that GSK3B, NRAS and ITGB1 were key genes. qRT-PCR results showed that Mmu-miR-124 was increased significantly and GSK3B was decreased slightly during the neurodevelopment. Conclusion Mmu-miR-124 is widely involved in a variety of biological processes by regulating target genes, and Mmu-miR-124 may regulate neural development by targeting GSK3B.

Key words: Mmu-mir-124, Target gene, Bioinformatics analysis, Neural development

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