组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2018, Vol. 14 ›› Issue (6): 353-356.doi: 10.3969/j.issn.1673-0364.2018.06.015

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脂肪血管基质组分获取方法及临床应用的研究进展

孙思捷，王克明，马继光   

  1. 中国医学科学院北京协和医学院整形外科医院整形十七科
  • 收稿日期:2018-09-15 修回日期:2018-11-03 发布日期:2020-07-23
  • 作者简介:马继光(E-mail:majiguang@psh.pumc.edu.cn)。

Research Progress of SVF Isolation and Clinical Application

SUN Sijie   

  • Received:2018-09-15 Revised:2018-11-03 Published:2020-07-23
  • Contact: 国家自然科学基金项目(81550025)

摘要： 脂肪血管基质组分(Stromal vascular fraction,SVF)是抽脂脂肪去除成熟脂肪细胞后剩余的多种细胞及基质成分的组合,主要包含脂肪来源干细胞(Adipose-derived stem cells,ADSCs)、造血干细胞、内皮祖细胞、巨噬细胞等。其中,ADSCs具有多向分化潜能,并可分泌多种细胞因子,在组织再生及修复中具有重要作用。SVF的获取方法主要包括酶消化法与机械乳化法,但获取标准尚未达成共识。SVF已广泛应用于辅助脂肪移植、面部年轻化、瘢痕及脱发治疗等整形外科领域,也可应用于放射性损伤、骨关节炎、糖尿病等疾病的治疗。目前关于SVF的安全性及临床效果仍存争议,需要基础实验及临床研究探索SVF的最佳获取方法及作用机制,提高SVF临床使用的安全性和疗效,并进一步扩大应用。

关键词: 基质血管组分, 脂肪来源干细胞, 纳米脂肪, 自体脂肪移植

Abstract: Stromal vascular fraction(SVF)is a heterogeneous collection of cells,which is the remaining ingredients after removing mature fat cells in the adipose tissue of transplantation.SVF includes adipose-derived stem cells(ADSCs),hematopoietic stem cell,endothelial precursor cells,macrophages,etc.ADSCs have multipotent differentiation capacity and can release of various cytokines thus show promise in regenarative and reconstructive medicine.The methods for acquiring SVF mainly include enzymatic and mechanical isolation,which have not reached a wide consensus.SVF has been widely used in plastic surgery including enhancing the viability of fat grafts,skin rejuvenation,treatment of facial scars and alopecia,etc.SVF is also comparably effective in several human diseases including radiation injuries,osteoarthritis,diabetes and so on.But there is still debate with regards to safe and efficacious use of SVF.Further studies are needed to investigate the optimal procedures for efficient SVF isolation,elucidate its characterization and discover newer applications apart from the plethora already identified.

Key words: Stromal vascular fraction, Adipose-derived stem cells, Nanofat, Autologous fat grafting

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