组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2019, Vol. 15 ›› Issue (6): 393-397.doi: 10.3969/j.issn.1673-0364.2019.06.005

• 论著 • 上一篇    下一篇

基于静电纺丝人脐带华通胶构建组织工程软骨的实验研究

李豪，徐勇，夏会堂，周广东，姜格宁，李承德   

  1. 潍坊医学院药理学教研室，山东省重点应用药理学实验室；上海交通大学医学院附属第九人民医院整复外科，上海市组织工程研究重点实验室；上海市肺科医院胸外科
  • 收稿日期:2019-09-20 修回日期:2019-11-13 出版日期:2019-12-20 发布日期:2019-12-20
  • 作者简介:李承德(E-mail:lichde@126.com);姜格宁(E-mail:jgnwp@aliyun.com)。

Experimental Research of Tissue Engineering Cartilage Based on Electrospun Human Umbilical Cord Wharton’s Jelly

LI Hao,XU Yong,XIA Huitang,ZHOU Guangdong,JIANG Gening,LI Chengde   

  1. Department of Pharmacology, Weifang Medical College;Department of Plastic and Reconstructive Surgery;Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine; Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine
  • Received:2019-09-20 Revised:2019-11-13 Online:2019-12-20 Published:2019-12-20
  • Contact: 国家自然科学基金项目(81570089)

摘要： 目的探索人脐带华通胶静电纺丝纳米纤维膜的制备及其作为软骨组织工程支架的可行性。方法人脐带华通胶经脱细胞处理后,与聚己内酯(PCL)共混制备成静电纺丝纳米纤维膜。取兔耳软骨细胞接种于该纤维膜支架,体外培养验证其细胞相容性;随后植入裸鼠体内培养,6周后行组织学检测,验证其体内成软骨的可行性。结果华通胶经脱细胞处理后去除了细胞成分,且保留了绝大部分胶原。华通胶/PCL混合后成功制备出多孔结构的纳米纤维膜。活死细胞染色和CCK-8细胞增殖曲线证实,该纤维膜具有良好的细胞相容性。体内培养6周后取材,组织学证实该纤维膜复合软骨细胞可再生成熟的软骨组织。结论人脐带华通胶可通过静电纺丝技术,制备成适用于软骨组织工程的支架材料。

关键词: 华通胶, 支架, 静电纺丝, 软骨再生

Abstract: Objective To explore the preparation of nanofiber membrane by human umbilical cord Wharton’s jelly and to explore its feasibility as a scaffold for cartilage tissue engineering. Methods The human umbilical cord Wharton’s jelly was decellularized and mixed with polycaprolactone(PCL) to prepare an electrospun nanofiber membrane. The cytocompatibility of the nanofiber membrane was verified by implantation with chondrocytes derived from rabbit ear in vitro. Thereafter, the chondrocytes-nanofiber membrane constructs were implanted in nude mice for 6 weeks to verify the feasibility of cartilage formation in vivo by histological examination. Results After decellularization of Wharton’s jelly, the cellular components were removed and most of the collagen was retained. The porous nanofiber membrane was successfully prepared by mixed Wharton’s jelly with PCL. Live and dead staining and CCK-8 assay confirmed that the nanofiber membrane possessed perfect cytocompatibility. In vivo histological observation confirmed that the nanofiber membrane-chondrocytes construct could regenerate mature cartilage tissue. Conclusion The human umbilical cord Wharton’s jelly can be fabricated into a suitable scaffold by electrospinning technology for cartilage tissue engineering.

Key words: Wharton’s jelly, Scaffold, Electrospun, Cartilage regeneration

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