组织工程与重建外科杂志 ›› 2017, Vol. 13 ›› Issue (1): 5-12.doi: 10.3969/j.issn.1673-0364.2017.01.002

• 论著 • 上一篇    下一篇

辣椒素抑制体外培养瘢痕疙瘩成纤维细胞增殖的实验研究

林苗苗,王文波,康文,高振,武晓莉,唐胜建,刘伟   

  1. 潍坊医学院整形外科研究所;上海交通大学医学院附属第九人民医院整复外科;上海组织工程研究重点实验室
  • 发布日期:2020-07-23

Inhibitory Effect of Capsaicin on the Proliferation of Keloid Fibroblasts Cultured in Vitro

LIN Miaomiao,WANG Wenbo,KANG Wen,GAO Zhen,WU Xiaoli,TANG Shengjian,LIU Wei   

  • Published:2020-07-23

摘要: 目的 探讨辣椒素对瘢痕疙瘩成纤维细胞增殖能力、胶原表达及迁移能力的影响,为辣椒素治疗瘢痕疙瘩提供依据。方法 取人瘢痕疙瘩9例,胶原酶消化获取瘢痕疙瘩成纤维细胞,用含有0.5μg/L和5μg/L辣椒素的DMEM培养液培养细胞,常规DMEM培养液培养作为对照。采用CCK-8法检测细胞增殖能力,流式细胞术检测周期变化,QPCR检测胶原和炎症因子等的表达水平,并以划痕实验检测细胞的迁移能力。结果辣椒素能抑制瘢痕疙瘩成纤维细胞的增殖,阻滞细胞于G0/G1期,并抑制Ⅰ、Ⅲ型胶原和纤连蛋白的表达。此外,辣椒素还能降低瘢痕疙瘩细胞炎性因子白细胞介素-6(IL-6)、白细胞介素-8(IL-8)及肿瘤坏死因子-α(TNF-α)的表达。但是,辣椒素对细胞的迁移能力没有影响。结论 辣椒素能抑制体外培养的瘢痕疙瘩成纤维细胞的增殖能力,并抑制其胶原和炎性因子等的表达。

关键词: 瘢痕疙瘩, 成纤维细胞, 辣椒素, 增殖

Abstract: Objective To explore the effect of capsaicin on inhibiting collagen expression, proliferation and migration ability of keloid fibroblasts in vitro and thus to provide scientific basis for clinical application of capsaicin in treatment of keloid. Methods The keloid tissues were harvested from 9 patients and digested with 0.3% collagenase to extract keloid fibroblasts. These cells were treated with different concentrations of capsaicin (0.5 μg/L and 5 μg/L) as the experiment group or without capsaicin treatment as the control group. CCK-8 assay was used to evaluate cell proliferation. Flow cytometry was also employed to evaluate cell cycle. Q-PCR assay was used to examine the gene expression levels of collagen and other extracellular matrices. In vitro wound scratch was also used to investigate cell migration ability. Results Capsaicin inhibited the proliferation of keloid fibroblasts, resulting in relative blockade of cell cycle in G0/G1 phase. It also inhibited the gene expression of collagens Ⅰ and Ⅲ and fibronectin. In addition, Capsaicin also inhibited the gene expression of IL-6, IL-8 and TNF-α, but had no effect on cell migration. Conclusion Capsaicin could inhibit the proliferation and gene expression of collagen and inflammatory cytokines of cultured fibroblasts in vitro.

Key words: Keloid, Fibroblasts, Capsaicin, Proliferation

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