组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2017, Vol. 13 ›› Issue (1): 9-12.doi: 10.3969/j.issn.1673-0364.2017.01.003

• 论著 • 上一篇    下一篇

苦参素抑制裸鼠人源增生性瘢痕的初步研究

王爱丽，徐顺，顾耀辉，黄静，贾卿，陈波   

  1. 上海中医药大学附属第七人民医院烧伤整形科
  • 发布日期:2020-07-23

The Inhibitory Effects of Oxymatrine Coating Agent on Human Hypertrophic Scar

WANG Aili,XU Shun,GU Yaohui,HUANG Jing,JIA Qing,CHEN Bo   

  • Published:2020-07-23
  • Contact: 上海市浦东新区卫计委科技发展专项基金面上项目；上海市中医、中西医结合临床重点扶持项目

摘要： 目的 探讨苦参素对裸鼠人源增生性瘢痕胶原合成和纤维化的抑制作用。方法 将人源增生性瘢痕移植到裸鼠背部(n=30),构建增生性瘢痕裸鼠模型。随机分为空白对照组、苦参素涂膜剂组和阳性药组,术后10 d开始分别涂抹空白涂膜剂、苦参素和醋酸曲安奈德涂膜剂,每天2次,连续8周。每周观察并拍照,记录瘢痕生长情况,于术后10 d,以及用药后35 d、49 d和56 d时测量瘢痕面积;HE染色观察组织形态;RT-PCR检测瘢痕中α-SM-Actin和Ⅰ、Ⅲ型胶原的表达。结果 涂抹药物35 d、49 d和56 d,苦参素涂膜剂组瘢痕面积显著缩小,与空白对照组和阳性药组相比差异显著(P<0.05);RT-PCR结果显示,与空白对照组相比,苦参素涂膜剂组和阳性药组的α-SM-Actin与Ⅰ、Ⅲ型胶原表达水平显著降低(P<0.05)。结论 苦参素涂膜剂对增生性瘢痕胶原合成有明显抑制作用,并具有抗瘢痕纤维化的作用。

关键词: 苦参素, 涂膜剂, 增生性瘢痕, 纤维化

Abstract: Objective To investigate the inhibitory effects of Oxymatrine coating agent on hypertrophic scar. Methods Human hypertrophic scar tissues were subcutaneously transplanted into the back of 30 nude mice to create the animal model of hypertrophic scar. Ten days after the operation, the mice were distributed into the control group, Oxymatrine coating agent group and positive medicine group, and were treated with blank coating agent, Oxymatrine coating agent and triamcinolone acetonide acetate coating agent respectively. Drugs were applied 2 times a day for 8 weeks. The growth of the scar was observed and photographed every week, and the size was measured 10 days after operation, and 35, 49, 56 days after drug treatment. HE staining was used for histological observation. RT-PCR and western blot assessment were used to detect the expression of Collagen Ⅰ, Ⅲ and α-SM-Actin. Results After drug treatment for 35, 49 and 56 days, the scar sizes in Oxymatrine coating agent group shrunk obviously and were much smaller compared with control group and positive medicine group (P<0.05). According to RT-PCR assessment, the expression of Collagen Ⅰ,Ⅲandα-SM-Actin were declined sharply in Oxymatrine coating agent group and positive medicine group, compared with the control group. Conclusion Oxymatrine coating agent has obvious inhibitory effects on hypertrophic scar, and also has anti-fibrosis effect.

Key words: Oxymatrine, Coating agent, Hypertrophic scar, Fibrosis

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