胰腺癌血清微RNA-486-3p的异常表达及对细胞增殖、凋亡的影响

doi:10.16138/j.1673-6087.2021.02.011

摘要/Abstract

摘要：

目的：研究胰腺癌患者血清微RNA（microRNA,miRNA/miR）-486-3p的表达情况及其在体外实验中对人胰腺癌细胞增殖、凋亡的影响。方法：于消化科、胰腺外科及老年科病房收集确诊胰腺癌患者21例为试验组,20名健康成人为对照组,留取外周血并分离血清,实时定量聚合酶链反应（polymerase chain reaction,PCR）检测血清中miR-486-3p的表达水平;体外实验采用细胞计数试剂盒8（cell-counting kit 8,CCK-8）法和流式细胞仪分别检测miR-486-3p对人胰腺癌细胞增殖、细胞凋亡的影响。结果：与健康对照组相比,胰腺癌患者血清中miR-486-3p的表达水平明显升高（50.73±0.82比34.80±0.74,P<0.05）; 体外实验中采用干扰小RNA（small interfering RNA,siRNA）瞬时转染的方法增高miR-486-3p的表达,使得人胰腺癌细胞SW1990和PANC-1的增殖能力明显增强（2.77±0.07比2.05±0.06,P<0.05;2.81±0.04比1.89±0.04,P<0.05）;抑制miR-486-3p则可明显减少胰腺癌细胞的增殖（1.71±0.03比2.07±0.05,P<0.05;1.61±0.03比2.20±0.07,P<0.05）;增高miR-486-3p的表达可明显减少细胞凋亡（24.1%±1.14%比45.9%±1.11%,P<0.05;21.9%±0.25%比42.3%±1.62%,P<0.05）。结论：胰腺癌患者血清中miR-486-3p较健康对照组明显升高,可促进胰腺癌细胞的增殖并抑制细胞凋亡而发挥促癌作用。

关键词: 胰腺癌, 血清微RNA-486-3p, 细胞增殖和凋亡

Abstract:

Objective To investigate the serum level of microRNA-486-3p (miR-486-3p) and its effects on proliferation and apoptosis of pancreatic cancer cells. Methods Twenty-one patients with pancreatic cancer and twenty healthy controls were enrolled. Real-time polymerase chain reaction(PCR) was used to detect the serum miR-486-3p. The effects of miR-486-3p on pancreatic cancer cells proliferation and apoptosis were respectively examined by cell counting kit 8(CCK8) assay and flow cytometry assay. Results Compared with healthy controls,serum level of miR-486-3p was significantly up-regulated in patients with pancreatic cancer （50.73±0.82 vs 34.80±0.74, P<0.05）; The proliferation of pancreatic cancer cells were significantly up-regulated （SW1990: 2.77±0.07 vs 2.05±0.06, P<0.05; PANC-1: 2.81±0.04 vs1.89±0.04, P<0.05） and the apoptosis rates were suppressed by forced expression of miR-486-3p（24.1%±1.14% vs 45.9%±1.11%, P<0.05; 21.9%±0.25% vs 42.3%±1.62%, P<0.05）, while inhibition of miR-486-3p significantly down-regulated the proliferation of pancreatic cancer cells（SW1990: 1.71±0.03 vs 2.07±0.05, P<0.05; PANC-1: 1.61±0.03 vs 2.20±0.07, P<0.05）. Conclusions The serum miR-486-3p were significantly up-regulated in patients with pancreatic cancer; MiR-486-3p may function as a tumor-promoter by promoting cell proliferation and suppressing apoptosis in pancreatic cancer cells.

Key words: Pancreatic cancer, Serum microRNA-486-3p, Cell proliferation and apoptosis

中图分类号:

R735.9

李晓丽, 李为光, 钱爱华, 曹国良. 胰腺癌血清微RNA-486-3p的异常表达及对细胞增殖、凋亡的影响[J]. 内科理论与实践, 2021, 16(02): 121-125.

LI Xiaoli, LI Weiguang, QIAN Aihua, CAO Guoliang. The serum level of microRNA-486-3p and its effects on proliferation and apoptosis of pancreatic cancer cells[J]. Journal of Internal Medicine Concepts & Practice, 2021, 16(02): 121-125.

图/表 2

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细胞	转染miR-486-3p 类似物		转染miR-486-3p 抑制剂
细胞	阴性对照	类似物	阴性对照	抑制剂
SW1990	2.05±0.06	2.77±0.07¹⁾	2.07±0.05	1.71±0.03¹⁾
PANC-1	1.89±0.04	2.81±0.04¹⁾	2.20±0.07	1.61±0.03¹⁾