组织工程与重建外科杂志 ›› 2017, Vol. 13 ›› Issue (1): 25-28.doi: 10.3969/j.issn.1673-0364.2017.01.007

• 论著 • 上一篇    下一篇

iRGD-外泌体-阿霉素抑制恶性黑色素瘤体外增殖的研究

赵莉娟,郑江红,柳向东,茅广宇,邓辰亮,杨松林   

  1. 上海交通大学附属第六人民医院整形外科
  • 发布日期:2020-07-23

The Inhibition of iRGD-Exosomes-Doxorubicin on the Proliferation of Malignant Melanoma in Vitro

ZHAO Lijuan,ZHENG Jianghong,LIU Xiangdong,MAO Guangyu,DENG Chenliang,YANG Songlin   

  • Published:2020-07-23

摘要: 目的 探讨应用i RGD-外泌体-阿霉素抑制人恶性黑色素瘤细胞系A375体外增殖的有效性及其靶向性。方法体外转染i RGD,并提取外泌体,通过电穿孔包裹阿霉素,利用流式细胞仪检测i RGD-外泌体-阿霉素对A375细胞的亲和力,并对i RGD-外泌体-阿霉素抑制A375细胞的增殖能力进行分析。结果 i RGD-外泌体-阿霉素与A375细胞的结合率高于空白转染-外泌体-阿霉素;i RGD-外泌体-阿霉素可明显抑制A375细胞的增殖,空白转染-外泌体-阿霉素未观察到明显的抑制情况。结论 i RGD-外泌体-阿霉素能通过靶向作用于A375细胞,并抑制其增殖。

关键词: 恶性黑色素瘤, 靶向治疗, 外泌体, 阿霉素

Abstract: Objective To investigate the effect and targeting of iRGD-exosomes-doxorubicin (iRGD-exo-dox) on the inhibition of A375 cell line. Methods The exosomes were extracted from the iRGD-transfected A375 cells. The doxorubicin was wrapped by electroporation. Flow cytometry was used to detect the affinity of iRGD-exo-dox to A375 cells. The ability of iRGD-exo-dox on inhibiting the proliferation of A375 cells was analyzed. Results The affinity of iRGD-exo-dox to A375 cells was higher than the blank-exo-dox. The proliferation of A375 cells was obviously inhibited by iRGD-exo-dox. The inhibition effect was not observed in blank-exo-dox group. Conclusion iRGD-exo-dox can inhibit the proliferation of A375 cells by targeting function.

Key words: Malignant melanoma, Targeted therapy, Exosome, Doxorubicin

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