组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2016, Vol. 12 ›› Issue (5): 285-288.doi: 10.3969/j.issn.1673-0364.a450

• 论著 • 上一篇    下一篇

种植体联合骨组织工程技术修复犬下颌骨节段缺损的实验研究

李东，毛豪丽，白珊珊，袁捷，韦敏，张文杰，刘伟，曹谊林   

  1. 上海交通大学医学院附属第九人民医院整复外科；上海市组织工程研究重点实验室；上海交通大学医学院附属第九人民医院麻醉科
  • 发布日期:2020-07-23

Research of Repairing Canine Segmental M andibular Bone Defects by Using Im p lant Combining w ith Bone Tissue Engineering

LIDong1,2,MAO Haoli3,BAIShanshan1,YUAN Jie1,WEIMin1,ZHANGWenjie1,2,LIUWei1,2,CAO Yilin1,2   

  1. 1 Department of Plastic and Reconstructive Surgery;2 Shanghai Key Laboratory of Tissue Engineering;3 Department of Anesthesiology,Shanghai Ninth People's Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011, China.Corresponding author:YUAN Jie E-mail:jaman1@126.com);ZHANGWenjie E-mail:wenjieboshi@yahoo.com.cn).
  • Published:2020-07-23
  • Contact: 国家自然科学基金（81471886）；上海市科委重点项目（134119a2000）。

摘要： 目的 观察应用种植体联合骨组织工程技术,修复犬下颌骨节段缺损的效果。方法 体外扩增培养、成骨诱导犬BMSCs。将第2代细胞复合珊瑚后修复犬自体右侧下颌骨3 cm的节段缺损,术后32周植入种植体(实验组n=3);同时,以邻近正常骨植入种植体作为对照(n=3)。植入4周、12周、26周后,分别通过影像学、大体形态观察、组织学和生物力学等方法,检测骨缺损的修复效果。结果 植入后26周,X线片和CT均显示种植体与实验组及对照组骨质为良好骨性愈合,实验组种植体周围新生骨密度较高。Micro-CT显示,实验组骨密度和对照组间无显著性差别(P>0.05)。大体观察见种植体与组织工程骨和正常骨均形成紧密连接。组织学显示,实验组与对照组均有较多成熟骨结构。生物力学测试结果表明,实验组与正常下颌骨力学强度无显著性差异(P>0.05)。结论 自体成骨诱导BMSCs复合珊瑚形成的组织工程化骨,可较好地修复犬下颌骨节段缺损,植入种植体后可进一步促进骨成熟。

关键词: 种植体, 下颌骨修复, 骨髓基质干细胞, 珊瑚

Abstract: Objective To investigate the effect of repairing canine segmentalmandibular bone defects by using implant combining with bone tissue engineering.M ethods A 3 cm segmentalmandibular defect was created,and the defect was repaired with cell-scaffold constructs formed by in vitro expanded and osteogenically induced BMSCs(P2)and coral. Implantswere embedded into themandible 32 weeks post-operation(n=3);the samewas disposed for the adjacentmandible as control(n=3).Imaging,gross view,histological and biomechanicalmethodswere taken to detect the repairing effects of the bone defect at 4 weeks,12 weeks,26 weeks respectively after implantation.Results A well implant-osseous healing was identified in both experimental group and control group by X-ray and computed tomography(CT)26 weeks after implantation,while the experimental group showed a higher neogenetic bone density around the implant.Micro-CT indicated no significant density difference between the experimental group and the control group(P＞0.05).Closely connection were formed between the implant and the normal bone as well as the tissue engineered bone.Histologically,lots ofmature bone trabecula was showed in both groups.No significant mechanical-strength difference was found in mandible between thebiomechanical testing group and the normal group(P＞0.05).Conclusion The tissue-engineered bone formed by autologous osteogenic induction of BMSCs and coral can be a favorable remediation of the caninemandible.Additionally,the implanted implant can further promote bonematuration.

Key words: Implant, Mandibular reparation, Bonemarrow stromal cells, Coral

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