组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2015, Vol. 11 ›› Issue (4): 221-224.doi: 10.3969/j.issn.1673-0364.2015.04.001

• 论著 • 上一篇    下一篇

Sox9诱导人脐血干细胞向软骨细胞分化的实验研究

张军，张耀明，王正辉，张青，罗花南，郑国玺，侯瑾，王波涛，杨叶叶，赵小燕，史艳霞   

  1. 延安大学附属医院耳鼻喉科二病区；延长县人民医院耳鼻喉科；西安交通大学第二附属医院耳鼻咽喉-头颈外科
  • 发布日期:2020-07-23

Chondrogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cells Induced by Sox9

ZHANG Jun,ZHANG Yaoming,WANG Zhenghui,ZHANG Qing,LUO Huanan,ZHENG Guoxi,HOU Jin,WANG Botao,YANG Yeye,ZHAO Xiaoyan,SHI Yanxia   

  • Published:2020-07-23
  • Contact: 国家自然科学基金资助项目(81000416)；中央高校基本科研业务费专项资金(西安交通大学国际合作类)；西安交通大学第二附属医院重点项目基金

摘要： 目的 探讨Sox9基因对脐血干细胞向软骨细胞分化的影响。方法 体外分离培养人脐血干细胞,采用脂质体转染Sox9载体质粒,观察转染后细胞形态的变化,免疫组化染色观察collagenⅡ、aggrecan的表达,RT-PCR检测collagenⅠ、collagenⅡ、aggrecan的m RNA水平变化,Western blot检测collagenⅡ的表达变化。结果 Sox9对脐血干细胞形态无明显影响,与传统方法诱导组相比,Sox9转染后可明显促进脐血干细胞向软骨细胞分化。结论 Sox9对脐血干细胞的软骨分化有很强的调控作用,脐血干细胞可作为组织工程软骨一种良好的种子细胞。

关键词: 脐血干细胞, 分化, Sox9基因

Abstract: Objective To investigate the effect of Sox9 gene on inducing the chondrogenic differentiation of human umbilical cord mesenchymal stem cells. Methods Human umbilical cord mesenchymal stem cells (hUCMSCs) were obtained by microdissection and digestion, and cultured in monolayer. The growth velocity and morphological changes of hUCMSCs were observed after Sox9 transfection. The expression of collagen Ⅱ and aggrecan were detected by immunohistochemistry. The mRNA expression of collagen Ⅰ, collagen Ⅱ and aggrecan were detected by RT-PCR, and collagen Ⅱ content was detected by western blot. Results The Sox9 gene had no significant effect on the morphology of hUCMSCs, and significantly accelerated the differentiation of hUCMSCs to chondrocytes compared with the traditional induction group. Conclusion Sox9 gene can regulate the differentiation of hUCMSCs, and hUCMSCs can be used as seed cells for cartilage tissue engineering.

Key words: Umbilical cord mesenchymal stem cells, Differentiation, SRY-related high mobility group-box gene 9

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