Abstract: Objective To explore the feasibility of hBMSCs acting as seeding cell for tissue engineered bone in the repairing of mandibular after tumor surgery.Methods Three patients with mandibular defect were treated by tissue engineered bone after tumor surgery.The hBMSC were isolated by percolling gradient centrifugation from patient''s bone marrow aspirated from iliac crest.The hBMSC were cultured in vitro and induced.The osteogenic induced hBMSC were in partly demineralized allogenic bone scaffold and cultured for 1 month.The defects were repaired with the cell-scaffold construct.All cases were followed up for half a month,3 months,2 years postoperatively by PET-CT.Results PET-CT demonstrated that tissue engineered bone was formed in 3 months postoperatively.The tissue engineered bone can repair bone defect,and maintained stable effect.Conclusion The tissue engineered bone from hBMSCS can be formed in human body stably and used for repairing mandibular defect with satisfactory results.

Key words: GetLinkList(KeywordFilter(''Bone marrow stromal cell(BMSC), Mandibular defect, Tissue engineered bone, Repair''),''kw'',''CJFQ'')