Abstract: Objective To study the effect of tenomodulin(TNMD) over-expression on the expression of tenocyte related molecules of NIH3T3 and C3H10T1/2 cell lines.Methods The NIH3T3 and C3H10T1/2 cells were cultured in vitro and then transfected with pCAGGS vector and pCAGGS-TNMD expression vector respectively.After confirmation of successful transfection by RT-PCR,the morphology of transfected cells were observed with inverted phase contrast microscope,and the expression of some tenocyte related genes in transfected cells were analyzed with Quantitative PCR.Results There were no obvious changes in the morphology of NIH3T3 and C3H10T1/2 cells after transfection.The gene expression of collagenⅥ and biglycan in NIH3T3 cells decreased dramatically,while that of collagenⅠdid not shown obvious change.However,in C3H10T1/2 cells,the expression of collagenⅠand biglycan increased dramatically respectively,collagenⅥ did not shown obvious difference.Conclusion Tenomodulin(TNMD) over-expression of NIH3T3 and C3H10T1/2 cell lines did not affect the cell morphology,but could affect the expression of some tenocyte related genes.

Key words: Tendon, Over-expression, Tenomodulin, NIH3T3, C3H10T1/2