Journal of Tissue Engineering and Reconstructive Surgery ›› 2014, Vol. 10 ›› Issue (6): 309-313.doi: 10.3969/j.issn.1673-0364.2014.06.003

• Original article • Previous Articles     Next Articles

Human Adipose-De rived Stem Cells and its Biocompatibility with Bladder Acellular Matrix Graft-Silk Fibroin Bilayer Scaffold

ZHAO Yang,WU Jiasheng,ZHOU Zhe,ZHOU Juan,ZHANG Ming,LI Wei,WANG Zhong,SUN Kang,LU Mujun   

  1. Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine;Shanghai Jiaotong University School of Materials Science and Engineering;
  • Published:2020-07-23
  • Contact: 国家自然科学基金项目(81070605,81370860);上海交通大学“医工(理)交叉研究基金”(YG2011MS14)

Abstract: Objective To observe the growth of human adipose-derived stem cells (hASCs) in bladder acellular matrix graft-silk fibroin (BAMG-SF) bilayer scaffold and to analyze the biological compatibility of BAMG-SF with hASCs. Methods hASCs were isolated from human subcutaneous adipose tissue after collagenase digesting, filtrating and centrifuging, then cultured in the leaching solution of BAMG-SF. The cytotoxicity of scaffold was evaluated by CCK-8 cell viability assay, and the growth curves were also observed. Surface morphology on BAMG-SF was observed by scanning electron microscopy (SEM). The hASCs of passage 3 were seeded onto the BAMG-SF bilayer scaffolds for 1 week, then the BAMG-SF bilayer scaffolds seeded with hASCs were transplanted into nude mouse for 1 week or 2 weeks. The growth of cells in BAMG-SF biomaterials was observed by HE staining. The species origin of these cells in the BAMG-SF scaffolds cultured in vivo was detected by Immunofluorescence. Results hASCs maintained high proliferation rate in the leaching solution of BAMG-SF and the BAMG-SF scaffolds were nontoxic absolutely. According to the growth curves of hASCs cultured in the leaching solution of the BAMG-SF and DMEM, BAMG-SF scaffolds were conducive to the growth of hASCs. The histological study found that hASCs could grow into the space of the BAMG-SF scaffolds after cultured in vitro and in vivo. There were more cells in the scaffolds cultured in vivo than in vitro. Immuno-fluorescence suggested that some of the cells inside the scaffolds were hASCs. Conclusion BAMG-SF bilayer scaffolds are nontoxic and have a good biocompatibility with hASCs, which can be used as a vehicle for hASCs in bladder defect reconstruction.

Key words: Human adipose-derived stem cells, Bladder acellular matrix graft, Silk fibroin, Bilayer scaffold, Tissue engineering

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