Journal of Tissue Engineering and Reconstructive Surgery ›› 2015, Vol. 11 ›› Issue (2): 90-94.doi: 10.3969/j.issn.1673-0364.2015.02.011

• Original article • Previous Articles     Next Articles

Comparison between Bone Marrow-derived Mononuclear Cells and Mesenchymal Stem Cells in Promoting Survival Rates of Random-pattern Skin Flap in Rats

XU Peng,TANG Zhengya,LU Yang,ZHOU Guangdong,LIU Wei,CAO Yilin,ZHANG Wenjie   

  1. Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering;National Tissue Engineering Center of China;
  • Published:2020-07-23
  • Contact: 国家重点基础研究发展计划（“973”项目）（2011cb964704）；国家自然科学基金（81271714,31170944）

Abstract: Objective To compare the non-cultured bone marrow mononuclear cells (BM-MNCs) and cultured bone marrow mesenchymal stem cells (BMSCs) in the promotion of random-pattern skin flap survival of rats. Methods BM-MNCs and BMSCs derived from two identical rat bone marrow aspirates were injected in a random-pattern skin flap model of rat (n=6). The survival area was determined by its appearance, color and texture. Capillary density of flaps was measured by histology. Results The flap survival rates were (71.6±8.4)%in the BM-MNC-treated group and (66.2±3.1)%in the BMSC-treated group, both of which were significantly higher than the control group (55.9 ±3.4)%. However, no significant difference was observed between the cell transplanted groups. According to vessel density assay, capillary density in the BM-MNC-treated group (58.2±6.8) was higher than in the BMSC-treated group (42.7±5.1), both of which were significantly higher than the control group (22.8±3.1). Conclusion Pre-culture of BMSCs does not bring therapeutic benefits.

Key words: Random-pattern skin flap, Cell therapy, Bone marrow mononuclear cells, Bone marrow mesenchymal stem cells

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