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23 July 2020, Volume 12 Issue 5 Previous Issue    Next Issue

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Two-D imensional Co-Culture of Bone M arrow Mesenchymal Stem Cells and Endothelial Cells for Inducing the Self-Assemb led Vascular Network ZHANG Lei,FU Wei,ZHANG Wen,BAI Jie,FENG Bei,TANG Ziqing, ZHANG Haibo. 2016, 12 (5):  269-280.  DOI: 10.3969/j.issn.1673-0364.a447 Abstract ( 420 )   Objective To observe the vascular network formation by co-culturing rat bone marrow mesenchymal stem cells(BMSCs)and endothelial cells(ECs)under conditions of two-dimensional co-culturing with/without serum,which would provide a minimum basal co-culture condition for the further study of the interactions between the cells.M ethods Rat BMSCs were isolated by direct adherence of the whole bonemarrow,and were identified by cellmorphology,cell surface antigens and three-lineage differentiation.Low-glucose DMEM with/without 10%FBSwas used during the co-culture of rat BMSCs and ECs,followed by a consecutive observation of the vascular network formation.CM-Dil solely labeled rat BMSCs or ECs were then co-cultured to verify the cell type within the network.Finally,immunofluorescent staining of EC marker CD31 and smoothmuscle cellmarker SM22αand Calponin was used to identify the cell differentiation during the co-culture. Resu lts Whole marrow direct adherence was used to isolate rat BMSCs successfully.With medium containing 10%FBS, co-cultured cells assembled vascular network in 8 days,which was formed together by rat BMSCs and ECs.In serum-free medium,however,self-assembled vascular network was formed together by co-cultured cells within 24 hours.In both settings,CD31+cells and SM22αor Calponin weremostly seen in the network structure.Conclusion Rat BMSCs and ECscan be co-cultured on two-dimensional plateswith/without serum-containingmedium for the induction of the self-assembled vascular network. Related Articles | Metrics

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Design and Fabrication of Abacterial Cellulose Bilayer Scaffold for Urethral Tissue Engineering LI Zhe,LV Xiangguo,WANG Baoxiu,FENG Chao,CHEN Shiyan,YAN Zhiyong,WANG Huaping. 2016, 12 (5):  276-280.  DOI: 10.3969/j.issn.1673-0364.a448 Abstract ( 307 )   Objective To design and fabrication of the 3D scaffolds that exhibit biomimetic multi-scale structures composed ofmicroporous structure with nanofibers for urethral tissue.Methods By analyzing themulti-scale structures of urethral acellularmatrix,a bilayer scaffold composed ofmicroporous section and nanofibrous dense section to biomimetic urethral tissue was designed.Template biosynthesis was used to fabricate 3D microporous nanofibrous gelatin/BC bilayer scaffolds(BC-Gel/BC)by stationary cultivation usingmicroporous gelatin scaffold as a template.Results The BC-Gel/BC bilayer scaffold composed of dense section of BC nanofibrils network and microporous section of Gel/BC.Themicroporous section with highly interconnected micropore(167±56)μm and surface decorated on themicropore wall by BC nanofibers (20-40)nm were fabricated,which were remarkably similar structure to the native extracellularmatrix(ECM).Conclusion Using the template biosynthesis method,the BC-Gel/BC bilayer scaffold for urethral tissue engineering can be successfully constructed,which has the bilayer structure,m icro porous and nanofibrous network to biom imetic urethral tissue. Related Articles | Metrics

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CAEM-COLⅡN anofibe r Scaffolds Com posite w ith BM SCs in Repairing Ar ticular Car tilage Defect of Rabbits JIANG Ting,YANG Zelong,LIXiaobin. 2016, 12 (5):  281-284.  DOI: 10.3969/j.issn.1673-0364.a449 Abstract ( 378 )   Objective To observe the effect of CAEM-COLⅡnanofiber scaffolds composite with BMSCs in repairing articular cartilage defectof rabbits.Methods Cartilage acellular extracellularmatrix(CAEM)and collagenⅡ(COLⅡ)were mixed according to the ratio of 1∶1,nanofiber scaffolds were prepared by electrostatic spinning for cartilage tissue engineering.The second generations of bonemarrow stem cells(BMSCs)were inoculated onto the scaffolds.The cell scaffold composite was placed in the incubator for two hours.12 Japanese white rabbitswere randomly divided into the experimental group and the control group.In the experimental group,the cell scaffold composite was implanted into the articular cartilage defects of rabbits.In the control group,only the knee joint cartilage defects weremade.After 12 weeks,the animals were sacrificed and the repair effect was observed by gross appearance,HE staining and collagen typeⅡstaining.Resu lts General shape observation showed that cartilage defects in the experimental group were repaired well,and were filled with granulation tissue in the control group.HE staining showed that cartilage cavities formed in articular cartilage defects in the experimental group,and fibrous tissues were filled in the control group.In the repair area,collagen typeⅡstaining was positive in the experimental group,and negative in the control group.Conclusion The CAEM-COLⅡnanofiber scaffolds composite with BMSCs could well repair the articular cartilage defects of rabbits,and have potential of application in tissue engineering cartilage. Related Articles | Metrics

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Research of Repairing Canine Segmental M andibular Bone Defects by Using Im p lant Combining w ith Bone Tissue Engineering LIDong,MAO Haoli,BAIShanshan,YUAN Jie,WEIMin,ZHANGWenjie,LIUWei,CAO Yilin 2016, 12 (5):  285-288.  DOI: 10.3969/j.issn.1673-0364.a450 Abstract ( 375 )   Objective To investigate the effect of repairing canine segmentalmandibular bone defects by using implant combining with bone tissue engineering.M ethods A 3 cm segmentalmandibular defect was created,and the defect was repaired with cell-scaffold constructs formed by in vitro expanded and osteogenically induced BMSCs(P2)and coral. Implantswere embedded into themandible 32 weeks post-operation(n=3);the samewas disposed for the adjacentmandible as control(n=3).Imaging,gross view,histological and biomechanicalmethodswere taken to detect the repairing effects of the bone defect at 4 weeks,12 weeks,26 weeks respectively after implantation.Results A well implant-osseous healing was identified in both experimental group and control group by X-ray and computed tomography(CT)26 weeks after implantation,while the experimental group showed a higher neogenetic bone density around the implant.Micro-CT indicated no significant density difference between the experimental group and the control group(P＞0.05).Closely connection were formed between the implant and the normal bone as well as the tissue engineered bone.Histologically,lots ofmature bone trabecula was showed in both groups.No significant mechanical-strength difference was found in mandible between thebiomechanical testing group and the normal group(P＞0.05).Conclusion The tissue-engineered bone formed by autologous osteogenic induction of BMSCs and coral can be a favorable remediation of the caninemandible.Additionally,the implanted implant can further promote bonematuration. Related Articles | Metrics

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The Clinical Comparison Study of Two Different Methods for Repairing Facial Soft Tissue Defect in Severe Progressive Facial Hem iatrophy CHEN Junbao,LI Binghang,TENG Li,LU Jianjian,XV Jiajie,ZHANG Chao,XIE Fang,YANG Liya,YANG Lu,LI Shuyuan,CAO Yilin 2016, 12 (5):  289-293.  DOI: 10.3969/j.issn.1673-0364.a451 Abstract ( 492 )   Objective To evaluate clinical effect of free anterolateral thigh adipofascial flap transplantation and autologous fatgraft to repair facial soft tissue defection in severe progressive facial hemiatrophy.Methods From September 2013 to December 2015,16 patients with progressive facial hemiatrophy were selected according to enter criterion.The patientswere divided into two groups according to different surgicalmethods,autologous fat graftgroup(Group A,n=8)and free anterolateral thigh adipofascial flap group(Group B,n=8).The facial three-dimensional data were acquired via 3-D scanner preoperatively.Using Mimics 17.0 and Geomagic studio 12.0 software,facial 3-Dmodel of patientwas reconstructed, facial symmetry was analyzed,the volume of facial tissue defect was calculated and the reconstruction scope of facial soft tissue was designed.All the patients were operated and clinical indexeswere analysed after 6 months.Results The facial symmetry and soft tissue defect in Group A and Group B 6 months post-operation were both significantly improved compared with pre-operation(P＜0.05),while therewas no statistical significance between group A and group B(P＞0.05).The patients′clinical indexes:total operation time,postoperative hospital stay in Group B were greater than in Group A,times of hospitalization of Group B surpassed Group A with statistical significance(P＜0.05).Facial 3-D deviation analysis:there was no statistical significance between group A and group B(P＞0.05).Satisfaction score:patients postoperative satisfaction in Group A was better than in Group B(P＜0.05).The third person and surgeon satisfaction:no significant difference betweenthe two groups(P＞0.05).Conclusion The clinical effectof autologous fatgraft in repairing facial soft tissue defectof severe PFHA patients is non-inferior to free anterolateral thigh adipofascial flap transplantation with good clinical effects and high satisfactory,and isworthy of clinical application. Related Articles | Metrics

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Digital Three-Dimensional Molding Technology in the Repair of the Complex Jaw Depression Jl Hang, WANG Sushen, LA NG Gang, ZHA NG Zhihua, H OU Chun, CHA I Gang, CHEN X iaojun, Y A NG X ianxian, MA Jianhua 2016, 12 (5):  294-296.  DOI: 10.3969/j.issn.1673-0364.a452 Abstract ( 346 )   Objective To explore the efficiency of digital three-dimensional？molding technology for the repair of the complex maxillofacial depression. Methods From January 2014 to May 2016, 12 cases with complex maxillofacial depression were treated using digital three-dimensional？molding technology. The surgical methods, timing and prognosis were analyzed retrospectively. Results Using the digital three-dimensional molding technology, the conformity has been greatly improved. 12 patients all regained their original shapes of maxillofacial bones. At the same time, the average operation time reduced by two hours. Up to now, no serious complications were observed, like shift, leakage or rejection of the complement. Conclusion Repairing maxillofacial depression with the technique of three-dimension shaping can obviously improve the conformity and reduce operation time and risk, which is worth popularization and application. Related Articles | Metrics

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Ear ly Repair of Deep W ound w ith E lectric Burn of Neck by Ped icled Latissimus Dorsi M yocutaneous Flap LIANG Zunhong,PAN Yunchuan,LIN Zhihu 2016, 12 (5):  297-299.  DOI: 10.3969/j.issn.1673-0364.a453 Abstract ( 369 )   Ob jective To explore the clinical effect of pedicled latissimus dorsimyocutaneous flap in early repairing deep electric burn wound of neck.M ethods From May 2010 to November 2015,5 patients with deep electric burn wound of neck were treated.Burn area ranged from 2%to 20%.After the early stage of debridement,the pedicled latissimus dorsi myocutaneous flap was used to repair the wound in the neck.Results All the flaps survived well with good texture and color.No necrosis or ulceration was observed.After followed up for 6 months to 2 years,the satisfied neck shape was achieved without movement limitation.Conclusion The neck wound of deep electric injury should be given early debridement and myocutaneous flap transfer;Latissimus dorsi flap is one of the good choice in repairing deep electrical burn wound of neck with constant vascular,abundantblood supply,sufficient donor area and flexible transfer. Related Articles | Metrics

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M odified N asolabial Rotation Flap in the Reconstruction of Upper Lateral L ip Subunit QIU Yajing,JIN Yunbo,LIN Xiaoxi,CHEN Hui,MANG Gang,YANG Xi,HU Xiaojie 2016, 12 (5):  300-301.  DOI: 10.3969/j.issn.1673-0364.a454 Abstract ( 367 )   Objective To exp lore the clinical efficacy ofmodified nasolabial rotation flap in the reconstruction of upper lateral lip subunit.Methods Ten patients suffering from large treatment-resistent portwine stains or hemangiomas of upper lipswere recruited,whose defect/upper lateral lip ration weremore than 50 percent.Modified nasolabial rotation flapswere used to reconstruct the upper lateral lip subunit.Results All flaps were survived.No flap loss was noted.All scars were hidden into the borders of the upper lateral lip subunit and were outlined by the philtrum column,nostril sill,alar base and nasolabial fold.The results were satisfactory.Conclusion Modified nasolabial rotation flaps based on upper lateral lip subunits are reliable for reconstructing large,superficial defects of the upper lateral lip aesthetically and functionally. Related Articles | Metrics

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Repair of Failed Nasal Reconstructions by Using the Upper Arm Skin Tube Method SUN Qingqing,LIU Kai 2016, 12 (5):  302-304.  DOI: 10.3969/j.issn.1673-0364.a455 Abstract ( 357 )   Objective To investigate amethod of repeated plastic reconstruction of the nose in patients after a failed or unsatisfactory initial reconstruction that totally destroyed the forehead tissue.Methods From January 2008 to January 2010, 3 cases with failed nasal reconstructions were repaired using the upper arm skin tube by four-stage.During StageⅠ,a skin tube was created using the internal aspectof the leftupper arm(18 cm×8 cm).During StageⅡ,which occurred three weeks after Stage I,the top end of the skin tube was severed and moved to the nasion.During StageⅢ,which occurred three weeks after Stage II,the skin tube was removed from the upper arm,forming a flap,which was trimmed to a three-lobed shape and folded inwards to construct the nasal alars,tip and columella.The seventh and eighth costal cartilageswere obtained to form an“L”-shaped scaffold.During StageⅣ,which occurred three weeks after StageⅢ,pedicle division and trimm ing were conducted.Resu lts The operation was successful in all three patients,and all the patientswere followed up for 1 to 2 years. The color and texture of the reconstructed noses were matched well to the surrounding skin,satisfactory appearance and ventilatory function were both achieved.Conclusion For patients with failed nasal reconstruction and destroyed forehead tissue or who are firmly against leaving amark in the forehead,the application of the upper arm skin tube for total nasal reconstruction is an effectivemethod. Related Articles | Metrics

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Staged Treatment Strategy for Asymmetric Bilateral Congenital Blepharoptosis JIANG Zhengzheng,LIU Fei, WANG Xi,YANG Qun,LUO Xusong,YANG Jun. 2016, 12 (5):  305-308.  DOI: 10.3969/j.issn.1673-0364.a456 Abstract ( 359 )   Objective To explore the staged treatment strategy for asymmetric bilateral congenital blepharoptosis. Methods From 2011 to 2013,30 cases of asymmetric bilateral congenital blepharoptosis were treated according to the staged strategy.At first stage,severe side(amountof descentover upper limbus:moderate to severe)was treated with levator aponeurosis resection and advancement combined with tarsus resection under general anesthesia.After 3 to 6 months,the contralateral side was treated with tarsus resection or levator aponeurosis resection under local anesthesia.All the patients were followed up for 3months to 2 years,ptosis correction and bilateral asymmetry weremeasured.Resu lts In total 60 eyes of 30 cases,sufficient correction was achieved in 37 eyes(61.7%),moderate correction in 23 eyes(38.3%).No under correction or overcorrection was observed.As to symmetry evaluation,23 cases(76.5%)showed good results and 7 cases (23.5%)showed fair results.No poor symmetry was observed.Conclusion Compared to our previous treatment experience, the staged treatment strategy can achieve better symmetry results for asymmetric bilateral congenital blepharoptosis. Related Articles | Metrics

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Com parison of Sim ply Topical Timolol 0.5%Cream and Combined with Intralesional Diprospan Injection for the Treatment of Thick Superficial Infantile Hemangiomas HUANG Huizhen,LIWei,YU Qian,MA Gang,QIU Yajing, CHANG Lei,JIN Yunbo,LIN Xiaoxi 2016, 12 (5):  309-312.  DOI: 10.3969/j.issn.1673-0364.a457 Abstract ( 531 )   Objective To compare the efficacy and safety of simply topical timolol 0.5%cream and timolol combined with intralesional Diprospan in the treatment of thick superficial infantile hemangiomas(IH).M ethods From January to November 2015,43 patients with thick superficial IH(2～6 mm)were recruited for a self-control study.Half lesion was treated with topical timolol 0.5%cream simply,while the other was treated with topical timolol 0.5%cream combined with Diprospan injection.Cream was applied four times daily for fivemonths,and the injection of Diprospan was given every 4 weeks.The injection time depended on tumor thickness and the effect,when the depth of lesion was less than 2 mm or no further improvementwas observed after three treatment procedures,injection treatment should be stopped.Finally,according to the thickness,the color and area score,the effective rate and the incidence rate of adverse reaction were compared between the two groups.Results Thirty-eight patients with thirty-night lesions finished the research.The efficacy rates of topical timolol group and combined treatment group were 92.3%and 97.4%,no significant difference was observed.The combined treatmentgroup exhibited better effect than timolol cream group,especially in the involution of thickness,color and onset time,while not in the involution of area.The side effects included ulceration(5.13%)and soft tissue atrophy(5.13%) were observed in combined treatment group and peeling(2.56%)in both group.Conclusion Compared with simply topical cream,intralesional Diprospan combined with topical timolol 0.5%cream has a better effect for treating superficial IH with a certain protruding degree in the aspects of thickness,color and onset time. Related Articles | Metrics

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Progress of Skin Substitutes for Regenerating Appendages LI Luyan,ZHANG Bing,LI Xiuyuan,MA Lie 2016, 12 (5):  313-330.  DOI: 10.3969/j.issn.1673-0364.a458 Abstract ( 378 )   It is still an urgent problem to regenerate and repair skin defects in clinic. Up to now, even though a lot of skin substitutes have been developed and realize the clinical application, most of them can only repair the structure of epidermis and simple dermis. The reconstructive skin tissue can＇t reconstruct the functions of normal skin, usually due to the lack of skin appendages, such as hair follicles, sweat glands and sebaceous glands. In this paper, from the aspects of seed cells, scaffold materials and its bioactivation, the skin substitutes with the ability of regenerating skin appendages, especially hair follicles were reviewed. Finally, some important challenges for the future studies in this field were discussed. Related Articles | Metrics

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The Research Progress and L im itations of Bone Tissue Engineering ZHOU Xiao,SHENG Xiaowu,ZENG Yong 2016, 12 (5):  319-321.  DOI: 10.3969/j.issn.1673-0364.a459 Abstract ( 671 )   The graft for repairing bone grafts can be categorized as autografts, allografts, and xenografts. However, each type of donor tissue comes with its own set of limitations. Tissue engineering strategies have been applied as promising alternatives to produce bone constructs that mimic the structure of natural bone and solve the limitations of bone defect treatment. In this paper, the research progress of bone tissue engineering was reviewed, and the limitations of current bone tissue approaches were discussed. Related Articles | Metrics

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GNAQ M utations in P ort-W ine S tains ZHU Jiafang,YUWenxin,MA Gang,LIN Xiaoxi 2016, 12 (5):  322-324.  DOI: 10.3969/j.issn.1673-0364.a460 Abstract ( 464 )   G-alpha-q （GNAQ） is the alpha subunit of the heterotrimeric GTP-binding proteins that mediates the regulators of several downstream signaling through coupling cell surface receptors. Recently, lesions within port-wine stains are found harbor mutations in GNAQ p.Arg183 and may at least associate with ERK （extracellular signal-regulated kinase） signaling pathways. In this paper, the mutations of GNAQ within port-wine stains and the multiple activated signaling targets downstream of mutant GNAQ, including MAPK, RGS5, PKC and YAP pathways were reviewed. Related Articles | Metrics

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The M easurement of Nasal M orphology and Its C linical App lication in Rhinop lasty WANG Yuting,PU zheming 2016, 12 (5):  325-327.  DOI: 10.3969/j.issn.1673-0364.a461 Abstract ( 367 )   Rhinoplasty is one of the most popular cosmetic operation in plastic surgery. But there is a wide variation between different ethnic group in nasal anatomy, morphologic measurement and esthetic value orientation. In this paper, based on the pertinent studies of nasal morphology which were published, the nasal anatomy and morphologic measurement of Asian female were summarized and analyzed, and compared with Caucasians, in order to provide the nasal measurement and facial aesthetics proportions for Asian patients inrhinoplasty. Related Articles | Metrics

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Botulinum Toxin Type A for Lower Facial Rejuvenation PAN benyun,WANG Danru 2016, 12 (5):  328-330.  DOI: 10.3969/j.issn.1673-0364.a462 Abstract ( 363 )   How to keep the finn lower facial contour along with the gradually aging process is the major point that Asian women focus on. They are seeking for an minimally invasive and less risky approach, compared with the traditional surgery. The past two decades has seen the botulinum toxin type A （BTX-A） injection as a relatively noninvasive, conservative, and reversible preferred method for women who seek for beauty. The application of BTX-A in upper and mid face has been widely accepted. The use of BTX-A in the lower face has been attracting more and more attention for harmonious lineament. In this paper, the research progress of Botulinum toxin type A for lower facial rejuvenation was reviewed. Related Articles | Metrics