Abstract: Objective To evaluate the feasibility and safety of co-transplantation of SVF-PLGA with fat complex on the construction of tissue engineered implant.Methods Forty adult New Zealand male rabbits were randomly divided into four groups.In the experimental group A(n=10),implant was constructed by use of SVF-PLGA with fat complex and in the group B (n=10).SVF-PLGA complex buried by adipose tissue was used.In the control group C(n=10) only PLGA and group D(n=10), SVF-PLGA complex was used.All the complex were translated between skin and superficial fascia.Tissue samples obtained were analyzed with gross observation,HE stain and red oil stain at 4 months postoperatively.Results The tissue engineered implant was mainly vascularized adipose tissue without tumor cell.PLGA scaffolds were degraded completely 4 months later. The survival rate of fat in experimental group B was higher than in the group A and in the control group B.Conclusion SVF-PLGA with fat complex on soft tissue with the advantage of high survival rate of fat.

Key words: Transplantation, Soft tissue, Implant, Tissue engineer, Scaffold, Fat