Journal of Tissue Engineering and Reconstructive Surgery ›› 2019, Vol. 15 ›› Issue (2): 107-110.doi: 10.3969/j.issn.1673-0364.2019.02.012

• Review • Previous Articles     Next Articles

modRNA Technology:A Newly-Developed Method of Protein Expression

YAN Bingqian,FU Wei   

  1. Institute of Pediatric Translational Medicine; Department of Pediatric Cardiothoracic Surgery,Shanghai Children's Medical Center,Shanghai Jiaotong University School of Medicine,Shanghai 200127,China;Shanghai Key Laboratory of Tissue Engineering,Shanghai 200011,China)
  • Received:2019-02-03 Revised:2019-03-16 Online:2019-04-20 Published:2019-04-20
  • Contact: 上海市浦江人才计划(18PJD031)；上海交通大学医工交叉项目(YG2016MS28)；上海交通大学医学院转化医学协同创新中心合作研究项目(TM201821)；上海交通大学医学院国际和地区联合研究项目；上海交通大学医学院紧缺专业硕士研究生临床研究能力提升计划(EKKY2018002DGD)

Abstract: Earlier in the 1990s,direct injection of naked mRNA has been proved to be an approach to achieve the exogenous gene expression in vivo.However,the instability and high-immunogenicity of mRNA becomes the main challenges of further development and application of this technology.Recently,with the in-depth research of mRNA structure and the advancement of nucleic acid technologies,chemically synthetic modified message RNA(modRNA)has emerged as a newly-developed gene vector,featuring stability,low-immunogenicity and high expression of target proteins.Based on the related researches,the concept,structure,mechanism and research status of modRNA were concluded,and the advantages and flaws along with the potential improvement direction were put forward in this paper.

Key words: Chemically synthetic modified message RNA, Gene expression, Mechanism

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