Abstract: Objective　To provide an animal model of staining agreement lymphaticovenular anastomosis (LVA) for training. Methods　The 2 mL methylene blue staining agent was injected into the brain tissue of experimental chicken. The experimental animals were killed in the state of hyperaemia of arteries and veins in the head and neck. After the head and body of the experimental animals were cleaned and wrapped with plastic bags, the neck region was shaved. A middle neck incision was made. After dissected to the carotid sheath position, lymphatic vessels were clearly exposed. The lymphatic vessels, lymph nodes and surrounding internal veins were dissected and separated under a microscope. End to end anastomosis, end to side anastomosis and lymph node vein anastomosis were performed under a 20-fold microscope. Each group was provided with 20 training animals. The training period lasted from 14 to 28 days, with an average of 21 days. Results　In all animal models after staining, clear stained lymphatic vessels can be found in the neck experimental area, and internal fat veins can be found in the surrounding fat, which could be used for trainees to carry out repeated anatomical separation and anastomosis training under the microscope. After the training, the trainees can achieve high patency rate of lymphatic venous anastomosis and lymph node venous anastomosis, and the immediate patency rate reached 80%. Conclusion　The training animal model made by this method is easy to obtain, the experimental cost is controllable, lymphatic staining is obvious in the experiment, and there is no stain contamination, so it is suitable for popularization and application of training animal model for LVA.

Key words: Animal model, 　Lymphatic venous anastomosis, 　Lymphatic staining