Journal of Tissue Engineering and Reconstructive Surgery ›› 2023, Vol. 19 ›› Issue (1): 28-.

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 Effect of non-ablative fractional laser combined with asiaticoside ointmenton hypertrophic scar of rabbit ear

  

  • Online:2023-02-01 Published:2023-03-08

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Abstract:

Objective　To explore the effect and mechanism of non-ablative fractional laser combined with asiaticoside
ointment on hypertrophic scar (HS) in rabbit ear. Methods　Rabbit ear HS model was established and divided into control group
(no modeling), model group (no intervention), combination group (1 565 nm non-ablative fractional laser combined with snow
side treatment), fractional group (1 565 nm non-ablative fractional laser treatment) and asiaticoside group (asiaticoside treatment)
according to different intervention methods. Gross observation, HE staining and MASSON staining were conducted to assess HS
improvement, fibroblast apoptosis was detected by TUNEL method. The expressions of type Ⅰ
and type Ⅲ collagen, TGF-β1,
Smads, IL-6 were detected by real-time PCR and Western blot. Results　After the intervention of the modeling group, gross
observation, HE staining and MASSON staining showed that scar hyperplasia was reduced in the combination group. TUNEL
showed that apoptotic cells were significantly increased in the combination group. Real-time and Western blot results showed the
mRNA and protein levels of type Ⅰ
collagen and type Ⅲ collagen, TGF-β1, IL-6 and Smad 2 in combination group increased,
while the expression of Smad 7 decreased. Conclusion　1 565 nm non-ablative fractional laser combined with asiaticoside
has significant effect in the treatment of HS, and is significantly better than that of laser or asiaticoside alone. Its mechanism is
related to the increased expression of TGF- β1, IL-6 and Smad 2, and the decreased expression of Smad 7.

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