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01 February 2023, Volume 19 Issue 1 Previous Issue   

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Biological effects of alginate oligosaccharides on wound: Degree of lysis dependence SHANG Siyu, XIANG Yang, PAN Bohan, et a 2023, 19 (1):  1.  Abstract ( 179 )   PDF (28656KB) ( 107 )   Objective　To explore the biological effects of alginate oligosaccharides with different degrees of lysis of wound cells and tissues. Methods　Alginate oligosaccharides were divided into five groups with molecular weight ranges of <500, 500-1 000, 1 000-5 000, 5 000-10 000, and 10 000-20 000 using dialysis bags with pores of different sizes. The cleavage degree was distinguished according to molecular weight, and the categories named experimental groups 1-5 respectively. Brown algal oligosaccharide (0.02% concentration) was used to co-culture with human keratinocytes and macrophages for 24 h, taking normal cultured cells as the control group. The cell proliferation ability of each group was evaluated, Caspase3 was detected via immunofluorescence to evaluate cell apoptosis, and an ROS kit was used to evaluate oxidative stress. After co-culture  with macrophages for 24 h, IL-1β, IL-10, and TNF- α were detected using ELISA and the endocytosis ability of the cells was observed using transmission electron microscopy. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of fucoidan against Staphylococcus aureus, Escherichia coli, and Candida albicans were detected using the micro-broth method. SD rats with a full-thickness skin defect wound model on their backs were treated with sodium alginate solution as positive control group, normal saline dressing was used as negative control group, and 0.02% brown algal oligosaccharide as the experimental group. Wound healing was observed on days 6 and 12, the wound tissue was stained with HE to observe histological changes, and inflammatory factors, apoptosis, and oxidative stress were detected. Results　At a fucoidan molecular weight of <500 or 500-1000, both the cell and animal experiments (on day 6) showed effective promotion of cell proliferation and IL-10 secretion, reduced IL-1 β, TNF- α, and apoptosis levels. Compared with the negative control group, the effect of promoting wound healing was more obvious when the molecular weight was less than 1 000, especially less than 500, but the effect of promoting wound healing was weaker than that of the positive control group (P <0.05). However, when the molecular weight of fucoidan was >1 000, the cell experiments showed no significant differences from the control group (P >0.05). In the animal experiment, when the molecular weight was 5 000-10 000 or 10 000-20 000, the wound healing rate increased (P <0.05). Compared with the positive control group, there was no significant difference in wound healing rate (P >0.05). In the experiment, it formed a thin membrane structure in the wound, but the other indicators did not change significantly (P >0.05). Enhanced phagocytosis was not observed by transmission electron microscopy. MIC and MBC detection of alginate oligosaccharides with various molecular weights did not reveal antibacterial or bactericidal effects at 5% concentration. Conclusion　The biological effects of fucoidan are strictly related to its molecular weight (cleavage degree). Low molecular weight fucoidan can improve the secretion of inflammatory factors, promote cell proliferation, inhibit cell apoptosis, and improve the degree of oxidative stress, which does not depend on whether fucoidan enters cells. High molecular weight fucoidan has no obvious biological effect, but forms a membrane and indirectly Related Articles | Metrics

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Application of integrated traditional Chinese and western medicine treatment plan in the wound repair after oral and maxillofacial head and neck tumor surgery LI Chaojun, WANG Yongling, LIAO Mingjuan, et al 2023, 19 (1):  10.  Abstract ( 122 )   PDF (5965KB) ( 96 )   Objective To explore the application value of the integrated traditional Chinese and Western medicine treatment plan in the woud repair of tissue defects after surgery for oral and maxillofacial head and neck tumors. Methods A total of 53 cases of post-operative wounds of oral and maxillofacial head and neck tumors were treated with traditional Chinese medicine changpi plaster combined with antibacterial dressings and other western medicine. The wound healing was observed after operation. Results In these cases, the shortest treatment time was 23 days, the longest treatment time was 1 year and 43 days. 35 cases were cured, 12 cases were markedly effective, 6 cases were ineffective. Conclusion The combination of traditional Chinese medicine and western medicine has obvious curative effect on postoperative wounds of oral and maxillofacial head and neck tumors, which can avoid multiple skin flap surgical repair, and is worthy of promotion. Related Articles | Metrics

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Study on the curative effect of personalized precise rehabilitation treatment strategy for secondary deformity after syndactyl operation YU Qinyuan, ZHOU Shengbo, ZHANG Mengyue, et al 2023, 19 (1):  14.  Abstract ( 101 )   PDF (15977KB) ( 66 )   Objective　To study the effect of personalized and precise rehabilitation diagnosis and treatment strategy on secondary deformities after congenital syndactyly. Methods A total of 40 children who met the inclusion criteria and were admitted to the Affiliated Yangzhi Rehabilitation Hospital of Tongji University from June 2019 to August 2021 were selected as the study objects and randomly divided into control group (n=20) and experimental group (n=20). The control group received intervention by traditional rehabilitation means. The experimental group used personalized precision rehabilitation treatment strategy for intervention. Before treatment, 4 weeks after treatment, 8 weeks after treatment and 12 weeks after treatment, the two groups of children were compared respectively in terms of scar, webbing, joint motion, hand function, family satisfaction of the children, and the incidence of adverse events during treatment. Results After 12 weeks of treatment, the scar condition, finger crawling, joint activity, hand function improvement, and family satisfaction of the experimental group were better than that of the control group, and the incidence of adverse reactions of the experimental group was lower than that of the control group, and the difference was statistically significant (P<0.05). Conclusion Personalized precise rehabilitation treatment strategy can play a significant role in improving the secondary malformation after surgery, significantly improving the scar condition of the child, finger crawling, joint activity, hand function, etc., while greatly improving the effect of postoperative rehabilitation, which is worth promoting. Related Articles | Metrics

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Efficacy analysis of‘core stripping’keloid thinning surgery combined with radiotherapy WU Xiaoli, BAN Yaolin, CHEN Libin, et al 2023, 19 (1):  22.  Abstract ( 185 )   PDF (9318KB) ( 76 )   Objective　To explore the effect of "core stripping" keloid thinning surgery combined with radiotherapy in the treatment of keloid. Methods　From January 2020 to December 2020, 43 patients with slope keloid and 326 keloid lesions were selected by retrospective study and self-control. The "core stripping" keloid thinning surgery was used to separate and remove the core tissue of keloid while retaining the scar skin with a thickness of about 1 mm. The reticular dermis was retained, and the scar skin was sutured and fixed by the "modified mattress suture method". After surgery, 6 Mev electron radiotherapy was performed twice. The first radiotherapy was completed within 24 hours after operation, and the second radiotherapy was performed 7 days later. The single dose of radiotherapy was 9 Gy. Follow-up was performed at 6 months, 12 months and 18 months after the end of treatment. The patient and observer scar scale (POSAS) was used to analyze the treatment outcomes, and  the treatment response rate and patients' satisfaction at 18 months after treatment were statistically analyzed. Results　The total score of POSAS, total score of patients, and total score of observers were significantly lower after treatment than before treatment. Some patients had different degrees of hyperpigmentation, hypopigmentation, local hair loss, transient dermatitis and neuritis, which improved spontaneously. The total recurrence rate at 18 months after treatment was 30.23%. Among 43 keloid patients, 17 patients (39.53%) were very satisfied with the treatment outcome, 22 patients (51.62%) were satisfied, and 4 patients (9.3%) were not satisfied. The overall satisfaction rate was 90.7%. Conclusion　The "core stripping" keloid thinning surgery is a rapid and effective keloid volume reduction surgery, which has a good therapeutic effect when combined with postoperative radiotherapy. Related Articles | Metrics

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Effect of non-ablative fractional laser combined with asiaticoside ointmenton hypertrophic scar of rabbit ear CHEN Xi, HAN Xiaofeng, XIAO Sha, et al 2023, 19 (1):  28.  Abstract ( 148 )   PDF (27469KB) ( 144 )   Objective　To explore the effect and mechanism of non-ablative fractional laser combined with asiaticoside ointment on hypertrophic scar (HS) in rabbit ear. Methods　Rabbit ear HS model was established and divided into control group (no modeling), model group (no intervention), combination group (1 565 nm non-ablative fractional laser combined with snow side treatment), fractional group (1 565 nm non-ablative fractional laser treatment) and asiaticoside group (asiaticoside treatment) according to different intervention methods. Gross observation, HE staining and MASSON staining were conducted to assess HS improvement, fibroblast apoptosis was detected by TUNEL method. The expressions of type Ⅰ and type Ⅲ collagen, TGF-β1, Smads, IL-6 were detected by real-time PCR and Western blot. Results　After the intervention of the modeling group, gross observation, HE staining and MASSON staining showed that scar hyperplasia was reduced in the combination group. TUNEL showed that apoptotic cells were significantly increased in the combination group. Real-time and Western blot results showed the mRNA and protein levels of type Ⅰ collagen and type Ⅲ collagen, TGF-β1, IL-6 and Smad 2 in combination group increased, while the expression of Smad 7 decreased. Conclusion　1 565 nm non-ablative fractional laser combined with asiaticoside has significant effect in the treatment of HS, and is significantly better than that of laser or asiaticoside alone. Its mechanism is related to the increased expression of TGF- β1, IL-6 and Smad 2, and the decreased expression of Smad 7. Related Articles | Metrics

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Preserved local flap combined with low dose radiotherapy for the treatment of ear keloid in Tibet region Bianba Laji, Ciwang Jiacuo, CAI Lei, et al 2023, 19 (1):  34.  Abstract ( 104 )   PDF (5716KB) ( 95 )   　Objective　To explore the effectiveness of local skin flap combined with low-dose radiation treatment for ear keloid in Tibet region. Methods　From April 2022 to August 2022, 8 patients with auricular keloid were treated with keloid resection under local anesthesia, local scar flap was retained, and local electron radiotherapy was combined after surgery in the plastic surgery Department of Xizang Autonomous Region People's Hospital. Results　The patients were followed up for 3-8 months (mean 5 months). All the 8 patients had stage I healing. Among the patients, 5 patients had flat surgical site without local symptoms, 2 cases had slight local tingling, 1 patient developed mild nodules in the operative area with pruritus 4 months after surgery, which were relieved spontaneously without special treatment. Conclusion　The use of local scar skin flap combined with radiation therapy can effectively treat ear keloid in Tibet region. Related Articles | Metrics

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Imaging features of intramuscular venous malformations: A single-center retrospective study GAO Dinghui, CHEN Yong, WANG Qian, et al 2023, 19 (1):  37.  Abstract ( 113 )   PDF (25070KB) ( 50 )   　Objective　To review the imaging features of intramuscular venous malformations (IMVMs) and to explore the guiding significance of imaging findings for the selection and implementation of treatment methods. Methods　From January 2006 to December 2021, 67 patients with IMVMs were treated in our department. The general data, clinical manifestations and imaging findings of the patients were retrospectively analyzed. The imaging methods were classified and evaluated, and the main diagnostic points of IMVMs were summarized. Individualized treatment strategies were proposed according to imaging features. Results　A total of 67 patients were included in this study, including 43 males. The patient complained of pain, swelling and limb dysfunction. The imaging examinations used included ultrasound, MRI/MRA, X-ray, CT/CTA/CTV, percutaneous angiography and three-dimensional CT imaging, etc. The above examination methods can show the location and morphology of the venous malformations, bony changes, draining features, hemodynamic characteristics, etc., so as to effectively guide the formulation of individual treatment strategies. Conclusion　The diagnosis of IMVMs depends on comprehensive and accurate imaging examinations, and the examination results are of guiding significance for the selection of appropriate treatment methods, the reasonable implementation of treatment, the avoidance of complications and the improvement of treatment effect. Related Articles | Metrics

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Preliminary clinical study of a single treatment with the unipolar focused radiofrequency device for skin type nasolabial folds LIU Jingqing, LIN Yanxian, YUAN Weiwei, et al 2023, 19 (1):  44.  Abstract ( 103 )   PDF (10918KB) ( 81 )   　Objective　To explore the efficacy and safety of a single high-energy treatment with the novel unipolar focused radiofrequency device for skin type nasolabial folds. Methods　A retrospective analysis was performed on 30 patients (26- 57 years old) diagnosed with skin type nasolabial folds from August to December 2021. The patients were given a single high energy treatment using the novel unipolar focused radiofrequency device. Photographs were taken before treatment and 3 months after treatment, the wrinkle severity rating scale (WSRS) was used by two doctors who did not participate in the treatment to evaluate the therapeutic effect before and 3 months after treatment, and the 5-point satisfaction questionnaire was filled in by the patients 3 months after treatment. Results WSRS score was significantly decreased 3 months after treatment compared with before treatment (P<0.05). 93.33% of the patients were satisfied with the therapeutic effect, and no serious adverse reactions were observed during or after the treatment. Conclusion A single high-energy treatment with the novel unipolar focused radiofrequency device is safe and effective for the correction of skin type nasolabial folds. Related Articles | Metrics

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Effect of compound preparation of barley protein and nicotinamide on facial rejuvenation XUANYUAN Xinyang, WANG Yutong, LIU Jianhang, et al 2023, 19 (1):  49.  Abstract ( 169 )   PDF (22357KB) ( 94 )   　Objective　To evaluate the efficacy of a topical compound of barley protein and nicotinamide in anti-wrinkle and facial brightening. Methods　From June to September 2020, 31 patients with a requirement for facial rejuvenation and skin lightening were included in the study. The topical compound of barley protein and nicotinamide was applied twice a day (2 drops in the morning and 4 drops in the evening). The changes in periocular wrinkles, skin elasticity and melanin index were evaluated at 4-week and 8-week follow-up. Results　After 8 weeks of treatment, 21 subjects showed significant improvement in the wrinkles around eyes, with an effective rate of 68%. VISIA showed that the number of facial wrinkles decreased significantly after 4 and 8 weeks of application (P<0.05). The R2 value of skin elasticity was significantly higher after 4 weeks of application (P<0.05). No treatment related side effects were observed. Conclusion　Topical application of barley protein and nicotinamide can lighten wrinkles and brighten skin. Related Articles | Metrics

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The status and influencing factors of anxiety and depression in patients with extracranial arteriovenous malformation TANG Ying, ZHOU Yanchun, CAO Xuanjun, et al 2023, 19 (1):  54.  Abstract ( 111 )   PDF (2768KB) ( 44 )   Objective　To investigate the state of anxiety and depression in patients with extracranial arteriovenous malformation (extracranial AVM) and analyze the influencing factors. Methods　From November 2020 to September 2021, 129 patients with extracranial AVM were selected to evaluate the anxiety and depression with Self-rating anxiety scale (SAS) and Self-rating depression scale (SDS), and the influencing factors were analyzed. Results　The SAS score and SDS score of 129 patients with extracranial AVM were 41.07±7.41 and 41.44±10.43. Education level, family relationship and sleep were important factors affecting anxiety and depression in patients with extracranial AVM (P=0.042 3, P=0.016 3, P=0.049 7). Conclusion　Patients with extracranial AVM have higher scores of anxiety and depression, and there are many influencing factors. Active measures should be taken to improve the status of anxiety and depression. Related Articles | Metrics

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Investigation of anxiety and depression in patients with hematological tumor and its correlation with treatment confidence  CHEN Ya, ZHOU Hui 2023, 19 (1):  59.  Abstract ( 106 )   PDF (1162KB) ( 52 )   Objective　To investigate the prevalence of anxiety and depression in the blood tumor patients, to analyze the relationship between treatment confidence and anxiety, depression, providing evidence for further improvement of nursing measures. Methods　Blood tumor patients in Shanghai Ninth hospital were investigated from January to March 2021. The anxiety and depression scale (HADS) was used to measure anxiety and depression scores, and the treatment confidence of patients was measured by numerical rating scale (NRS). Results　In all, 82 patients completed investigation. The majority of respondents had confidence in treatment (53/82). Among respondents investigated, 31.7% of them were suspected of anxiety, 12.2% of them were definitely with anxiety, 11.0% were suspected of depression, 4.9% of them were definitely with depression. There was significant correlation between treatment confidence and anxiety, depression in patients with hematologic tumors (P<0.01). Conclusion Anxiety and depression are common in patients with hematological tumor. The occurrence of anxiety and depression is significantly related to the lack of confidence in treatment. Therefore, corresponding intervention measures are needed Related Articles | Metrics

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#br# Nursing care of patients with facial and cervical scar treated with expanded superficial temporal island flap combined with laser hair removal XIA Fang 2023, 19 (1):  61.  Abstract ( 85 )   PDF (1059KB) ( 60 )   　Objective　To explore the nursing methods for patients with facial and neck scar treated by expanded superficial temporal island flap combined with laser hair removal. Methods　From January 2015 to December 2018, 10 patients with facial and neck scars were treated with expanded superficial temporal island flap combined with laser hair removal. During the treatment, psychological care, skin care, posture care, wound care and laser care were given. Results　A total of 10 cases were reviewed in this group. After postoperative nursing, only 1 case had postoperative blood circulation disorder, which belonged to venous reflux disorder. After 48 hours of intermittent acupuncture and bloodletting, blood circulation of the flap gradually stabilized. After 1 year follow-up, all the 10 flaps survived completely, with good appearance and improved function of the face and neck. Conclusion　For the treatment of facial and neck scar with expanded superficial temporal island flap combined with laser hair removal, timely and effective perioperative nursing and laser hair removal treatment can effectively reduce the occurrence of complications. Related Articles | Metrics

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Efficacy of ultrasound-guided femoral nerve block combined with infiltration between the popliteal artery and capsule of the posterior knee block (IPACK) in analgesia after anterior cruciate ligament reconstruction QIU Tao, WANG Peng, LI Yang, et al 2023, 19 (1):  64.  Abstract ( 202 )   PDF (1211KB) ( 82 )   　Objective　To investigate the efficacy of ultrasound-guided femoral nerve block (FNB) combined with infiltration between the popliteal artery and capsule of the posterior knee block (IPACK) in analgesia after anterior cruciate ligament reconstruction. Methods　From January 2021 to March 2022, 80 patients undergoing anterior cruciate ligament reconstruction were selected and divided into two groups by random number table method. The control group was treated with ultrasound-guided FNB, and the observation group was treated with ultrasound-guided FNB combined with IPACK block. The dosage of sufentanil, the number of PCIA compressions, the proportion of salvage analgesia, the degree of postoperative pain, the range of motion of knee joint, the muscle strength of lower limb, the longest walking distance, the leaving bed time and the adverse reactions of the two groups were compared. Results　The dosage of sufentanil, times of PCIA pressing, proportion of salvage analgesia and the leaving bed time in the observation group were all lower than those in the control group (P<0.05). Postoperative VAS scores at rest and activity were gradually decreased in both groups (P<0.05), postoperative knee joint range of motion, triceps calf muscle strength, quadriceps femoris muscle strength and the longest walking distance were gradually increased (P<0.05). VAS score of T0-4 activity in the observation group was lower than that in the control group (P<0.05), the knee joint range of motion and longest walking distance were higher than those in the control group (P<0.05). There were no statistically significant differences in VAS score at rest, triceps muscle strength and quadriceps muscle strength between the two groups (P>0.05). There was no significant 作者单位 ：236800　安徽省亳州市　亳州市人民医院麻醉科。? 65 ? 组织工程与重建外科 2023 年 2 月 第 19 卷第 1 期 difference in the incidence of complications between the two groups (P>0.05). Conclusion　Compared with FNB, ultrasound guided FNB combined with IPACK has a satisfactory analgesic effect after anterior cruciate ligament reconstruction, which is conducive to early postoperative rehabilitation and exercise Related Articles | Metrics

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Research progress of skin soft tissue expansion WEI Kunchen, XIE Yun, LI Qingfeng 2023, 19 (1):  70.  Abstract ( 121 )   PDF (1142KB) ( 162 )   　As an extremely important clinical method in plastic reconstruction, skin soft tissue expansion is widely used in the treatment of large-area skin defects. However, insufficient expansion efficiency and related complications limit its development. Plenty of researches have studied the molecular mechanism and potential regulatory targets related to skin expansion, and also explored how to improve the expansion efficiency and reduce the incidence of related complications. In this paper, the research progress related to skin soft tissue expansion was reviewed, and some possible development directions of this technology in the future were suggested. Related Articles | Metrics

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Research progress of signal pathways in soft palate development  ZHAO Jingjing, WU Di 2023, 19 (1):  75.  Abstract ( 83 )   PDF (1131KB) ( 247 )   　Cleft palate can adversely affect speech, swallowing, breathing and hearing. The latest progress in the study of soft palate development shows that the development of soft palate may be regulated by different pathways related to the development of hard palate. It is necessary to study the signal pathways involved in the development of soft palate. The soft palate includes three cell types: epithelial cells, cranial nerve crest-derived mesenchymal cells and muscle-derived cells, which interact with each other. In this paper, the research progress of soft palate development signal pathway in recent years was reviewed, in order to improve our understanding of the regulation mechanism of soft palate development, and provide a basis for improving the treatment of cleft soft palate in the future. Related Articles | Metrics

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Controlled release immunosuppression therapy in vascularized composite allotransplantation DENG Zhizhong, ZHOU Xianyu, LIU Fei, et al 2023, 19 (1):  79.  Abstract ( 99 )   PDF (1138KB) ( 156 )   　Vascularized composite allotransplantation (VCA) is an effective treatment to restore functional and aesthetic outcomes for patients suffering from devastating composite tissue loss. Life-long immunosuppression administration is usually mandatory for VCA patients to avoid immune rejection. However, the subsequent adverse effects would impair life quality or even cause death. Local delivery of controlled-release immunosuppressants is advantageous on maintaining a high level of medication and decreasing organ toxicities. In this paper, the biomaterial-based controlled-release systems in VCA were reviewed. Related Articles | Metrics

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Research progress of flap blood flow monitoring methods  SONG Zhen, LIN Guangxian, FAN Fei 2023, 19 (1):  83.  Abstract ( 121 )   PDF (1138KB) ( 96 )   Reconstruction of defects using flap is a common method in plastic and reconstructive surgery, and the survival of the flap is the key to the success of the operation. The design of the skin flap before and during the operation needs to consider the condition of the flap blood supply. Postoperative monitoring of the flap blood flow is helpful for early detection and treatment of flap blood flow problems, avoiding vascular crisis and flap necrosis happened. Therefore, flap blood flow monitoring is crucial to the survival of the flap. In this paper, the flap blood flow monitoring methods currently used in clinical and animal experiments, in order to provide a reference for the choice of flap blood flow monitoring methods in clinical and animal experiments Related Articles | Metrics

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Progress of free vascularized omental lymph node transplantation in the treatment of lymphedema of extremities CHENG Gechang, TANG Juyu 2023, 19 (1):  88.  Abstract ( 121 )   PDF (1061KB) ( 81 )   In recent years, with the increase number of patients with limb lymphedema and the development of super microsurgery, the treatment level of limb lymphedema is also improving. The donor site selection of transplanted lymph nodes is also being optimized. Body surface lymph nodes such as groin lymph nodes, axillary lymph nodes, submandibular lymph nodes and other donor areas have been widely used in the treatment of limb lymphedema. In this paper, the current status of free vascularized omental lymph node transplantation in the treatment of limb lymphedema with omental lymph nodes as donor area was reviewed. Related Articles | Metrics

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Research progress on buttock aesthetics and fat embolism events related to buttock augmentation by fat transplantation IU Anna, HUANG Zonglin, ZHU Congxiao, et al 2023, 19 (1):  91.  Abstract ( 182 )   PDF (1150KB) ( 94 )   As one of the most important methods of buttock cosmetic surgery, gluteal augmentation with fat grafting no longer simply emphasizes on the increase of hip volume, but more concerns about shaping plump, harmonious and smooth hip curve. Besides, the regional unitization and refinement of hip aesthetics can effectively improve the aesthetic effect of fat graft buttock augmentation. In addition, being a powerful technique used in gluteal augmentation, autogenous fat grafting has the advantages of small trauma, quick recovery and satisfactory results without obvious scar, however, its development is limited by relevant fat embolism events. How to improve the safety of surgery by treating and preventing fat embolism events is of great concern to plastic surgeons. In this paper, the research progress on buttock aesthetics and fat embolism events related to buttock augmentation by fat transplantation was reviewed. Related Articles | Metrics

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Teaching design and exploration of dermatology under the background of curriculum ideological and political construction YANG Yali, HUANG Linting, CHEN Jun, et al 2023, 19 (1):  97.  Abstract ( 457 )   PDF (9550KB) ( 99 )   According to the characteristics of dermatology and venereology, teachers are encouraged to explore the effective combination of medical professional courses with ideology and politics. Taking Acne as an example, the ideological and political elements in dermatology and venereal disease teaching were excavated, the organic integration of medical professional courses and ideological and political education was realized, which could improve students' humanistic quality and social responsibility awareness and emphasize the vital role of classrooms in ideological and political education. Related Articles | Metrics