Abstract: Objective　To evaluate the effect of autologous epidermal cell suspension combined with mesh skin graft in deep II-degree burn wounds. Methods　Patients with deep partial thickness burns were selected as the research subjects and randomly divided into two groups， control group and experimental group， according to the random table method. Control group received split-thickness mesh skin graft（ n=8）， and experimental group received autologous epidermal cell suspension combined with split-thickness mesh skin graft（ n=8）. The epidermal cell suspension was prepared from autologous donor skin by cell separator. The wound healing time， wound healing rate on first week after treatment， and scar score （Vancouver scale） on 8th week after surgery were statistically analyzed in the two groups. At the same time， the concentration and viability of epidermal cells in the cell suspension were detected. Results　The healing time of experimental group was （10.2±0.3） days， compared with（ 12.1±0.6） days in control group（ P<0.05）. The healing rates of control group and experimental group were 76.97%±10.68% vs. 94.85%±10.01% （P<0.05） one week after treatment， and the Vancouver scar scores of control group and experimental group were 6.13±3.44 vs. 2.92±1.07 （P<0.05） at 8 weeks after surgery. The concentration of cell suspension was（ 1.77±0.11）×106 cells per cm² skin sample， and the cell viability was 87.85%±4%. Conclusion　This study preliminarily confirmed that autologous epidermal cell suspension combined with mesh skin transplantation in the treatment of deep partial thickness burns can promote skin grafting healing time， reduce donor area， and improve postoperative scar hyperplasia.

Key words: Burns, 　Skin grafting, 　Autologous epidermal cell suspension