Abstract: Objective To investigate the effects of preoperative chemotherapy with cisplatin and 5-fluorouracil on abdominal flap in dogs.Methods Twenty-one male mongrel dogs were randomly divided into 3 groups(n=7): chemotherapy group,control group and blank control group.The dogs were treated with cisplatin(DDP,50 mg/m2) and 5-fluorouracil(5-Fu,150 mg/m2) in chemotherapy group.Chemotherapeutic drugs were intravenously injected on the 1,3 5 day.Each cycle went for 21 days,a total of 2 cycles.The dogs only received an injection of same amount of saline and glucose in control group,while nothing in the blank control group.The flap surgery was performed 42-49 days after injection and the changes of the flaps were observed after operation.On the 30th day after operation,the flap survival area was measured by means of coordinate transformations,then the entire flap was removed for pathological analysis.Results A total of 17 dogs survived(5 in chemotherapy group,6 in control group and 6 in blank control group).In the 2-3 days post operation,different degrees of swelling was observed in each flap.All flaps survived 30 days after operation.The survival area was similar among chemotherapy group,control group and blank control group(P=0.910).The epidermis and subcutaneous tissue grew well observed by light microscope.No obvious thrombosis and inflammatory cells were found in the inferior epigastric artery and veins in dogs of three groups.Conclusion Two cycles of preoperative chemotherapy with cisplatin and 5-fluorouracil has no significant influence on the survival area of abdominal flap.

Key words: Neoadjuvant chemotherapy, Flap, Cisplatin, 5-Fluorouracil